Detailed information of TA system TA172369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4627872..4628474
• Replicon: chromosome
• Accession: NZ_CP061329
• Organism: Escherichia coli strain EC93

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H8529_RS22100
• Protein ID: WP_000897305.1
• Coordinates: 4628163..4628474 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H8529_RS22095
• Protein ID: WP_000356397.1
• Coordinates: 4627872..4628162 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8529_RS22070	4623798..4624700	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H8529_RS22075	4624697..4625332	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H8529_RS22080	4625329..4626258	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
H8529_RS22085	4626588..4626830	-	243	WP_001087409.1	hypothetical protein	-
H8529_RS22090	4627049..4627267	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H8529_RS22095	4627872..4628162	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H8529_RS22100	4628163..4628474	-	312	WP_000897305.1	hypothetical protein	Toxin
H8529_RS22105	4628703..4629611	+	909	WP_001162704.1	alpha/beta hydrolase	-
H8529_RS22110	4629675..4630616	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H8529_RS22115	4630661..4631098	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H8529_RS22120	4631095..4631967	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H8529_RS22125	4631961..4632560	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T172369 WP_000897305.1 NZ_CP061329:c4628474-4628163 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T172369 NZ_CP079736:3435175-3435393 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT172369 WP_000356397.1 NZ_CP061329:c4628162-4627872 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT172369 NZ_CP079736:3434775-3435149 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7