Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3801924..3802465 Replicon chromosome
Accession NZ_CP061329
Organism Escherichia coli strain EC93

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag H8529_RS18360 Protein ID WP_000615976.1
Coordinates 3802187..3802465 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H8529_RS18355 Protein ID WP_000729704.1
Coordinates 3801924..3802184 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H8529_RS18330 3797707..3798492 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
H8529_RS18335 3798437..3800176 + 1740 WP_001438980.1 flagellar biosynthesis protein FlhA -
H8529_RS18340 3800281..3800559 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H8529_RS18345 3800552..3800908 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H8529_RS18350 3800965..3801738 - 774 WP_000543899.1 C40 family peptidase -
H8529_RS18355 3801924..3802184 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H8529_RS18360 3802187..3802465 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H8529_RS18365 3802621..3803361 + 741 WP_001225679.1 murein L,D-transpeptidase -
H8529_RS18370 3803332..3804099 - 768 WP_000333380.1 class II glutamine amidotransferase -
H8529_RS18375 3804305..3804883 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T172367 WP_000615976.1 NZ_CP061329:3802187-3802465 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T172367 NZ_CP079736:2656463-2656570 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT172367 WP_000729704.1 NZ_CP061329:3801924-3802184 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT172367 NZ_CP079736:c2656416-2656350 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References