Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604509..605308 Replicon chromosome
Accession NZ_CP061329
Organism Escherichia coli strain EC93

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag H8529_RS02925 Protein ID WP_000347267.1
Coordinates 604509..604973 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H8529_RS02930 Protein ID WP_001307405.1
Coordinates 604973..605308 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H8529_RS02895 599510..599944 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
H8529_RS02900 599962..600840 - 879 WP_001380079.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
H8529_RS02905 600830..601609 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
H8529_RS02910 601620..602093 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H8529_RS02915 602116..603396 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H8529_RS02920 603645..604454 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H8529_RS02925 604509..604973 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H8529_RS02930 604973..605308 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H8529_RS02935 605457..607028 - 1572 WP_001273741.1 galactarate dehydratase -
H8529_RS02940 607403..608737 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H8529_RS02945 608753..609523 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 604509..615964 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T172351 WP_000347267.1 NZ_CP061329:c604973-604509 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T172351 NZ_CP079735:c4090318-4090145 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT172351 WP_001307405.1 NZ_CP061329:c605308-604973 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172351 NZ_CP079735:c4090642-4090346 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References