Detailed information of TA system TA172347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 651055..651674
• Replicon: chromosome
• Accession: NZ_CP061319
• Organism: Polynucleobacter sp. AM-7D1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GQ359_RS03380
• Protein ID: WP_215387545.1
• Coordinates: 651055..651378 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GQ359_RS03385
• Protein ID: WP_114652461.1
• Coordinates: 651375..651674 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ359_RS03355 (GQ359_03355)	647555..648313	+	759	WP_215387541.1	molybdate ABC transporter substrate-binding protein	-
GQ359_RS03360 (GQ359_03360)	648351..648680	+	330	WP_215387542.1	metalloregulator ArsR/SmtB family transcription factor	-
GQ359_RS03365 (GQ359_03365)	648677..649156	+	480	WP_215387543.1	arsenate reductase ArsC	-
GQ359_RS03370 (GQ359_03370)	649153..650205	+	1053	WP_215387544.1	ACR3 family arsenite efflux transporter	-
GQ359_RS03375 (GQ359_03375)	650206..650925	+	720	WP_256442531.1	arsenical resistance protein ArsH	-
GQ359_RS03380 (GQ359_03380)	651055..651378	+	324	WP_215387545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GQ359_RS03385 (GQ359_03385)	651375..651674	+	300	WP_114652461.1	helix-turn-helix transcriptional regulator	Antitoxin
GQ359_RS03390 (GQ359_03390)	651701..651937	-	237	WP_215387546.1	hypothetical protein	-
GQ359_RS03395 (GQ359_03395)	652008..652433	-	426	WP_215387547.1	universal stress protein	-
GQ359_RS03400 (GQ359_03400)	652544..653317	-	774	WP_215387548.1	TSUP family transporter	-
GQ359_RS03405 (GQ359_03405)	653433..654326	+	894	WP_215387550.1	pseudouridine synthase	-
GQ359_RS03410 (GQ359_03410)	654334..655416	+	1083	WP_215387552.1	tRNA dihydrouridine(20/20a) synthase DusA	-
GQ359_RS03420 (GQ359_03420)	655618..656388	+	771	WP_215387553.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-95)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12487.77 Da
• Isoelectric Point: 9.7310

>T172347 WP_215387545.1 NZ_CP061319:651055-651378 [Polynucleobacter sp. AM-7D1]
MTYTIRYYSPDVLEQIFELPISLQARYIALTQRMIEYGPHLGLPHTDSFGGGLFELRLKGIEGIARVFFCTVVEREIVML
HSFIKKSQKTPDKELRLAKLRMREVKL

Nucleotide

• Length: 324 bp

>T172347 NZ_CP079735:c3627742-3627344 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10803.68 Da
• Isoelectric Point: 11.0229

>AT172347 WP_114652461.1 NZ_CP061319:651375-651674 [Polynucleobacter sp. AM-7D1]
MKTKTLTHKQAVSKMLKNPAVKAEVERLNKEEFAILDEILQARKAAGLSQAEVARRMGTQAPAIARLESSLATGKHSPSL
STLRKYAAALGKRVELHLV

Nucleotide

• Length: 300 bp

>AT172347 NZ_CP079735:c3628038-3627745 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA