Detailed information of TA system TA172320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 329894..330496
• Replicon: chromosome
• Accession: NZ_CP061302
• Organism: Polynucleobacter sp. MG-Unter2-18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C2759_RS01745
• Protein ID: WP_215355789.1
• Coordinates: 329894..330172 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2759_RS01750
• Protein ID: WP_215355790.1
• Coordinates: 330194..330496 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2759_RS01720 (C2759_01715)	325147..326166	-	1020	WP_215355780.1	ionic transporter y4hA	-
C2759_RS01725 (C2759_01720)	326257..326625	+	369	WP_215355782.1	CbiX/SirB N-terminal domain-containing protein	-
C2759_RS01730 (C2759_01725)	326548..327426	-	879	WP_215355783.1	uroporphyrinogen-III C-methyltransferase	-
C2759_RS01735 (C2759_01730)	327423..327971	-	549	WP_215355785.1	DUF934 domain-containing protein	-
C2759_RS01740 (C2759_01735)	327990..329729	-	1740	WP_215355787.1	nitrite/sulfite reductase	-
C2759_RS01745 (C2759_01740)	329894..330172	+	279	WP_215355789.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2759_RS01750 (C2759_01745)	330194..330496	+	303	WP_215355790.1	HigA family addiction module antitoxin	Antitoxin
C2759_RS01755 (C2759_01750)	330764..331081	+	318	WP_215355792.1	hypothetical protein	-
C2759_RS01760 (C2759_01755)	331144..331620	+	477	WP_215355793.1	helix-turn-helix domain-containing protein	-
C2759_RS01765 (C2759_01760)	331613..332437	+	825	WP_215355795.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C2759_RS01770 (C2759_01765)	332447..332818	-	372	WP_215355797.1	hypothetical protein	-
C2759_RS01775 (C2759_01770)	332846..333073	-	228	WP_215355799.1	hypothetical protein	-
C2759_RS01780 (C2759_01775)	333101..334009	-	909	WP_215355801.1	amidohydrolase family protein	-
C2759_RS01785 (C2759_01780)	334025..335035	-	1011	WP_215355803.1	tripartite tricarboxylate transporter substrate binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-90)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10645.24 Da
• Isoelectric Point: 9.3047

>T172320 WP_215355789.1 NZ_CP061302:329894-330172 [Polynucleobacter sp. MG-Unter2-18]
MIYSFSCKETQALFHSKTSRKFKNFERIARRKLLQLHAATNLLDLRVPPGNLLEALHGDRAGQHSIGINGQWRICFIWQV
DGVHEVEIVDHH

Nucleotide

• Length: 279 bp

>T172320 NZ_CP079725:c4782555-4782271 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11613.66 Da
• Isoelectric Point: 6.4708

>AT172320 WP_215355790.1 NZ_CP061302:330194-330496 [Polynucleobacter sp. MG-Unter2-18]
MTKLLDEIHPGEILLEDFMRPMEITARKLSADIDVSPSRISDIVHGNRPITADTAIRLGLFFGMDPKFWLNLQMEYDMRV
AKRILQKEIEPRIRVFQMAA

Nucleotide

• Length: 303 bp

>AT172320 NZ_CP079725:c4782787-4782545 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA