Detailed information of TA system TA172311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 551454..552073
• Replicon: chromosome
• Accession: NZ_CP061297
• Organism: Polynucleobacter asymbioticus strain MWH-MoIso1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AOC19_RS02840
• Protein ID: WP_215377413.1
• Coordinates: 551454..551777 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOC19_RS02845
• Protein ID: WP_114652461.1
• Coordinates: 551774..552073 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOC19_RS02815 (AOC19_02820)	547994..548746	+	753	WP_215377404.1	molybdate ABC transporter substrate-binding protein	-
AOC19_RS02820 (AOC19_02825)	548784..549110	+	327	WP_215377406.1	helix-turn-helix domain-containing protein	-
AOC19_RS02825 (AOC19_02830)	549110..549589	+	480	WP_215377408.1	arsenate reductase ArsC	-
AOC19_RS02830 (AOC19_02835)	549586..550638	+	1053	WP_215377410.1	ACR3 family arsenite efflux transporter	-
AOC19_RS02835 (AOC19_02840)	550639..551358	+	720	WP_215377411.1	arsenical resistance protein ArsH	-
AOC19_RS02840 (AOC19_02845)	551454..551777	+	324	WP_215377413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOC19_RS02845 (AOC19_02850)	551774..552073	+	300	WP_114652461.1	helix-turn-helix transcriptional regulator	Antitoxin
AOC19_RS02850 (AOC19_02855)	552102..552338	-	237	WP_215377415.1	hypothetical protein	-
AOC19_RS02855 (AOC19_02860)	552409..552834	-	426	WP_215377416.1	universal stress protein	-
AOC19_RS02860 (AOC19_02865)	552947..553720	-	774	WP_215377418.1	TSUP family transporter	-
AOC19_RS02865 (AOC19_02870)	553835..554728	+	894	WP_215378046.1	pseudouridine synthase	-
AOC19_RS02870 (AOC19_02875)	554736..555818	+	1083	WP_215377420.1	tRNA dihydrouridine(20/20a) synthase DusA	-
AOC19_RS02880 (AOC19_02885)	556022..556792	+	771	WP_215377422.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-95)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12433.67 Da
• Isoelectric Point: 9.7314

>T172311 WP_215377413.1 NZ_CP061297:551454-551777 [Polynucleobacter asymbioticus]
MTYTISYYSPDVLEQIFDLPISLQARYIALTQRMIEYGPHLGLPHTDSFGSGLFELRLKGIEGIARVFFCTVVEREIVML
HSFIKKSQKTPNKELRLAKLRMREVKL

Nucleotide

• Length: 324 bp

>T172311 NZ_CP079720:c895229-894879 [Heyndrickxia oleronia]
TTGATTGTTAAGCGTGGTGACGTGTATTTTGCTGACCTTTCCCCAGTTGTCGGATCTGAACAAGGTGGAGTCCGCCCTGT
TTTGGTCATTCAAAACGACATTGGGAATCGGTTTAGTCCCACTGTAATTGTTGCTGCTATTACAGCGCAAATCCAAAAAG
CAAAACTTCCTACACATGTTGAAATTGATGCAAAGCGTTATGGCTTTGAACGCGATTCTGTTATTTTGCTTGAACAAATT
CGCACCATTGATAAACAACGCTTAACAGACAAAATCACACATTTAGACGAAGAAATGATGGAAAAAGTGGACGATGCACT
GCAAATTAGTTTAGGACTTATTGAATTTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10803.68 Da
• Isoelectric Point: 11.0229

>AT172311 WP_114652461.1 NZ_CP061297:551774-552073 [Polynucleobacter asymbioticus]
MKTKTLTHKQAVSKMLKNPAVKAEVERLNKEEFAILDEILQARKAAGLSQAEVARRMGTQAPAIARLESSLATGKHSPSL
STLRKYAAALGKRVELHLV

Nucleotide

• Length: 300 bp

>AT172311 NZ_CP079720:c895515-895234 [Heyndrickxia oleronia]
GTGTCTGAATCCAGCGCAACAACAGAAATATTAATTCGGTTACCAGTACATTTATTATCAGAACTTGATGGATTTGCTGA
GCAAGAGAATGTGAATCGAAATGAATTTATTTATCGAGCTACAAAGATGTATCTTCGTGAGCGTAAAAAAAGACAGATTC
GCGAATCTATGAGACGCGGCTACATGGAAATGGCTAAAATTAATTTAGCAATAGCATCTGAGGCGATTCAAGCAGAGTAC
GAAGCAGAACATACAGTAGAACGCTTAGTAAGCGGGGGTTAG