Detailed information of TA system TA172261

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58376..58802
• Replicon: plasmid pDA61218-116
• Accession: NZ_CP061207
• Organism: Escherichia coli strain DA61218

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ICI53_RS24620
• Protein ID: WP_001312861.1
• Coordinates: 58376..58534 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	58578..58802 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI53_RS24590	53773..54462	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
ICI53_RS24595	54649..55032	-	384	WP_001151538.1	relaxosome protein TraM	-
ICI53_RS24600	55353..55955	+	603	Protein_69	transglycosylase SLT domain-containing protein	-
ICI53_RS24605	56252..57073	-	822	WP_001234445.1	DUF945 domain-containing protein	-
ICI53_RS24610	57183..57479	-	297	WP_001272251.1	hypothetical protein	-
ICI53_RS24615	57871..58075	+	205	Protein_72	pilus protein	-
ICI53_RS24620	58376..58534	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	58578..58802	-	225	NuclAT_0	-	Antitoxin
-	58578..58802	-	225	NuclAT_0	-	Antitoxin
-	58578..58802	-	225	NuclAT_0	-	Antitoxin
-	58578..58802	-	225	NuclAT_0	-	Antitoxin
ICI53_RS24625	58614..58793	+	180	WP_001309233.1	hypothetical protein	-
ICI53_RS24630	58814..59533	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
ICI53_RS24635	59530..59964	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
ICI53_RS24640	60033..61997	-	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
ICI53_RS24645	62058..62291	-	234	WP_000006003.1	DUF905 family protein	-
ICI53_RS24650	62349..62876	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
ICI53_RS24655	63178..63621	+	444	WP_024187997.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-48 / sul1 / qacE / ant(3'')-Ia	senB	1..116466	116466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T172261 WP_001312861.1 NZ_CP061207:c58534-58376 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T172261 NZ_CP079692:2048505-2048607 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 225 bp

>AT172261 NZ_CP061207:c58802-58578 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin