Detailed information of TA system TA172257

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16070..16323
• Replicon: plasmid pDA61218-116
• Accession: NZ_CP061207
• Organism: Escherichia coli strain DA61218

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: ICI53_RS24370
• Protein ID: WP_001336447.1
• Coordinates: 16070..16219 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	16267..16323 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI53_RS24335	12094..12495	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
ICI53_RS24340	12428..12685	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
ICI53_RS24345	12778..13431	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
ICI53_RS24350	13529..13669	-	141	WP_001333237.1	hypothetical protein	-
ICI53_RS24355	14370..15227	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
ICI53_RS24360	15220..15294	-	75	WP_001365705.1	RepA leader peptide Tap	-
ICI53_RS24365	15528..15785	-	258	WP_000084404.1	replication regulatory protein RepA	-
ICI53_RS24370	16070..16219	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	16267..16323	+	57	NuclAT_1	-	Antitoxin
-	16267..16323	+	57	NuclAT_1	-	Antitoxin
-	16267..16323	+	57	NuclAT_1	-	Antitoxin
-	16267..16323	+	57	NuclAT_1	-	Antitoxin
ICI53_RS24375	16418..16855	-	438	WP_000872609.1	hypothetical protein	-
ICI53_RS24380	17008..17391	-	384	WP_001109264.1	hypothetical protein	-
ICI53_RS24385	17494..17955	-	462	WP_000760080.1	thermonuclease family protein	-
ICI53_RS24390	18708..18911	-	204	WP_001336517.1	hypothetical protein	-
ICI53_RS24395	19063..19620	-	558	WP_000139329.1	fertility inhibition protein FinO	-
ICI53_RS24400	19675..20421	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-48 / sul1 / qacE / ant(3'')-Ia	senB	1..116466	116466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T172257 WP_001336447.1 NZ_CP061207:c16219-16070 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T172257 NZ_CP079685:c48955-48539 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT172257 NZ_CP061207:16267-16323 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin