Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3292135..3292862 Replicon chromosome
Accession NZ_CP061206
Organism Escherichia coli strain DA61218

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag ICI53_RS15430 Protein ID WP_000547555.1
Coordinates 3292135..3292446 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag ICI53_RS15435 Protein ID WP_000126293.1
Coordinates 3292443..3292862 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICI53_RS15400 3287276..3288985 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
ICI53_RS15405 3288995..3289537 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
ICI53_RS15410 3289537..3290304 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
ICI53_RS15415 3290301..3290711 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
ICI53_RS15420 3290704..3291174 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
ICI53_RS15425 3291217..3291972 + 756 WP_001305268.1 hypothetical protein -
ICI53_RS15430 3292135..3292446 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
ICI53_RS15435 3292443..3292862 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
ICI53_RS15440 3292954..3293382 - 429 WP_000536064.1 hypothetical protein -
ICI53_RS15445 3293767..3294294 + 528 WP_001078777.1 electron transport protein HydN -
ICI53_RS15450 3294447..3296699 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T172243 WP_000547555.1 NZ_CP061206:3292135-3292446 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T172243 NZ_CP079673:c3369914-3369696 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT172243 WP_000126293.1 NZ_CP061206:3292443-3292862 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT172243 NZ_CP079673:c3370315-3369941 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References