Detailed information of TA system TA172238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1900393..1900995
• Replicon: chromosome
• Accession: NZ_CP061206
• Organism: Escherichia coli strain DA61218

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: ICI53_RS08825
• Protein ID: WP_000897302.1
• Coordinates: 1900684..1900995 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ICI53_RS08820
• Protein ID: WP_000356397.1
• Coordinates: 1900393..1900683 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI53_RS08795	1896466..1897368	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
ICI53_RS08800	1897365..1898000	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ICI53_RS08805	1897997..1898926	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ICI53_RS08810	1899142..1899360	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
ICI53_RS08815	1899756..1900034	-	279	WP_001296612.1	hypothetical protein	-
ICI53_RS08820	1900393..1900683	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ICI53_RS08825	1900684..1900995	-	312	WP_000897302.1	hypothetical protein	Toxin
ICI53_RS08830	1901225..1902133	+	909	WP_001305059.1	alpha/beta hydrolase	-
ICI53_RS08835	1902197..1903138	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ICI53_RS08840	1903183..1903620	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ICI53_RS08845	1903617..1904489	-	873	WP_000920762.1	virulence factor BrkB family protein	-
ICI53_RS08850	1904483..1905082	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T172238 WP_000897302.1 NZ_CP061206:c1900995-1900684 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T172238 NZ_CP079673:c1755802-1755434 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT172238 WP_000356397.1 NZ_CP061206:c1900683-1900393 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT172238 NZ_CP079673:c1756020-1755799 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: S1P416