Detailed information of TA system TA172217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 2366881..2367417
• Replicon: chromosome
• Accession: NZ_CP061189
• Organism: Piscirickettsia salmonis strain Ps12201A

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6I5YBD1
• Locus tag: ICC15_RS12510
• Protein ID: WP_016211910.1
• Coordinates: 2366881..2367153 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: ICC15_RS12515
• Protein ID: WP_032126795.1
• Coordinates: 2367157..2367417 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICC15_RS12465	2362373..2362600	-	228	Protein_2443	transposase	-
ICC15_RS12470	2362596..2362748	+	153	WP_155046643.1	hypothetical protein	-
ICC15_RS12475	2362796..2363803	-	1008	WP_036774189.1	IS3 family transposase	-
ICC15_RS20175	2363803..2363940	-	138	WP_155097723.1	hypothetical protein	-
ICC15_RS12485	2364170..2364991	+	822	Protein_2447	IS30 family transposase	-
ICC15_RS12490	2365108..2365572	+	465	WP_036774631.1	hypothetical protein	-
ICC15_RS12495	2365631..2365771	-	141	Protein_2449	helix-turn-helix domain-containing protein	-
ICC15_RS12500	2365935..2366081	+	147	WP_157894761.1	transposase zinc-binding domain-containing protein	-
ICC15_RS12505	2366453..2366590	-	138	Protein_2451	helix-turn-helix domain-containing protein	-
ICC15_RS12510	2366881..2367153	-	273	WP_016211910.1	Txe/YoeB family addiction module toxin	Toxin
ICC15_RS12515	2367157..2367417	-	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ICC15_RS12520	2367690..2368280	+	591	WP_032126152.1	site-specific integrase	-
ICC15_RS12525	2368344..2369024	+	681	WP_016211531.1	MBL fold metallo-hydrolase	-
ICC15_RS12530	2369378..2370274	+	897	WP_016211530.1	Abi family protein	-
ICC15_RS12535	2370280..2370789	-	510	WP_016211534.1	hypothetical protein	-
ICC15_RS12540	2370776..2371726	-	951	WP_016211532.1	dCTP deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2313520..2425891	112371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10739.22 Da
• Isoelectric Point: 7.8926

>T172217 WP_016211910.1 NZ_CP061189:c2367153-2366881 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQ

Nucleotide

• Length: 273 bp

>T172217 NZ_CP079655:c4934429-4934145 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT172217 WP_032126795.1 NZ_CP061189:c2367417-2367157 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT172217 NZ_CP079655:c4934661-4934419 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A6I5YBD1

Antitoxin

• AlphaFold DB: A0A0B8U9U2