Detailed information of TA system TA172210

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 117698..118119
• Replicon: plasmid pLD67-1-165kb
• Accession: NZ_CP061188
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: IB283_RS25405
• Protein ID: WP_001302184.1
• Coordinates: 117698..117856 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	117921..118119 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS25365	112763..112978	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
IB283_RS25370	113114..113761	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
IB283_RS25375	113953..114336	-	384	WP_001063020.1	relaxosome protein TraM	-
IB283_RS25380	114688..115278	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
IB283_RS25385	115573..116394	-	822	WP_001234475.1	DUF945 domain-containing protein	-
IB283_RS25390	116513..116800	-	288	WP_000107546.1	hypothetical protein	-
IB283_RS25395	116825..117031	-	207	WP_000547965.1	hypothetical protein	-
IB283_RS25400	117101..117460	+	360	Protein_115	hypothetical protein	-
IB283_RS25405	117698..117856	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	117921..118119	-	199	NuclAT_0	-	Antitoxin
-	117921..118119	-	199	NuclAT_0	-	Antitoxin
-	117921..118119	-	199	NuclAT_0	-	Antitoxin
-	117921..118119	-	199	NuclAT_0	-	Antitoxin
IB283_RS25410	118131..118850	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
IB283_RS25415	118847..119281	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
IB283_RS25420	119336..121294	-	1959	WP_188322782.1	ParB/RepB/Spo0J family partition protein	-
IB283_RS25425	121360..121593	-	234	WP_000005990.1	DUF905 family protein	-
IB283_RS25430	121650..122189	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
IB283_RS25435	122507..123016	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..165427	165427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T172210 WP_001302184.1 NZ_CP061188:c117856-117698 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T172210 NZ_CP079655:335831-336199 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT172210 NZ_CP061188:c118119-117921 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin