Detailed information of TA system TA172207

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79544..79798
• Replicon: plasmid pLD67-1-165kb
• Accession: NZ_CP061188
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: IB283_RS25175
• Protein ID: WP_001351576.1
• Coordinates: 79544..79750 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	79737..79798 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS25150	74826..77831	+	3006	WP_001143760.1	Tn3-like element Tn3 family transposase	-
IB283_RS25155	77764..78229	-	466	Protein_66	incFII family plasmid replication initiator RepA	-
IB283_RS25160	78222..78704	-	483	WP_001273588.1	hypothetical protein	-
IB283_RS25165	78697..78771	-	75	WP_001442103.1	RepA leader peptide Tap	-
IB283_RS25170	79003..79260	-	258	WP_000083845.1	replication regulatory protein RepA	-
IB283_RS25175	79544..79750	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	79737..79798	+	62	NuclAT_1	-	Antitoxin
-	79737..79798	+	62	NuclAT_1	-	Antitoxin
-	79737..79798	+	62	NuclAT_1	-	Antitoxin
-	79737..79798	+	62	NuclAT_1	-	Antitoxin
IB283_RS25180	80054..80128	-	75	Protein_71	endonuclease	-
IB283_RS25185	80374..80586	-	213	WP_001299730.1	ANR family transcriptional regulator	-
IB283_RS25190	80722..81282	-	561	WP_000139315.1	fertility inhibition protein FinO	-
IB283_RS25195	81385..82245	-	861	WP_000704513.1	alpha/beta hydrolase	-
IB283_RS25200	82304..83050	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..165427	165427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T172207 WP_001351576.1 NZ_CP061188:c79750-79544 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T172207 NZ_CP079646:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT172207 NZ_CP061188:79737-79798 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin