Detailed information of TA system TA172196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4217124..4217923
• Replicon: chromosome
• Accession: NZ_CP061185
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: IB283_RS19815
• Protein ID: WP_000347273.1
• Coordinates: 4217459..4217923 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: IB283_RS19810
• Protein ID: WP_001308975.1
• Coordinates: 4217124..4217459 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS19795	4212909..4213679	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
IB283_RS19800	4213695..4215029	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
IB283_RS19805	4215404..4216975	+	1572	WP_077516436.1	galactarate dehydratase	-
IB283_RS19810	4217124..4217459	+	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
IB283_RS19815	4217459..4217923	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
IB283_RS19820	4217978..4218787	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
IB283_RS19825	4219036..4220316	+	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
IB283_RS19830	4220339..4220812	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
IB283_RS19835	4220823..4221602	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
IB283_RS19840	4221592..4222470	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
IB283_RS19845	4222488..4222922	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T172196 WP_000347273.1 NZ_CP061185:4217459-4217923 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T172196 NZ_CP079644:c50926-50510 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT172196 WP_001308975.1 NZ_CP061185:4217124-4217459 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT172196 NZ_CP079644:c51153-50923 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08