Detailed information of TA system TA172179

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2151827..2152198
• Replicon: chromosome
• Accession: NZ_CP061185
• Organism: Escherichia coli strain LD67-1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: IB283_RS09985
• Protein ID: WP_001317028.1
• Coordinates: 2152004..2152198 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2151827..2152005 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB283_RS09955	2147579..2147752	+	174	WP_001296046.1	hypothetical protein	-
IB283_RS09960	2147782..2149155	+	1374	WP_022645642.1	ATP-dependent RNA helicase DbpA	-
IB283_RS09965	2149284..2150219	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
IB283_RS09970	2150271..2151506	-	1236	WP_000040858.1	site-specific integrase	-
IB283_RS09975	2151508..2151723	-	216	WP_000079604.1	excisionase XisR	-
IB283_RS09980	2151802..2152011	-	210	WP_021565074.1	double-strand break reduction protein RcbA	-
-	2151827..2152005	+	179	NuclAT_0	-	Antitoxin
-	2151827..2152005	+	179	NuclAT_0	-	Antitoxin
-	2151827..2152005	+	179	NuclAT_0	-	Antitoxin
-	2151827..2152005	+	179	NuclAT_0	-	Antitoxin
IB283_RS09985	2152004..2152198	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
IB283_RS09990	2152255..2153064	-	810	WP_000166319.1	recombination protein RecT	-
IB283_RS09995	2153057..2155657	-	2601	WP_047667559.1	exodeoxyribonuclease VIII	-
IB283_RS10000	2155759..2156034	-	276	WP_000632297.1	protein RacC	-
IB283_RS10005	2156109..2156279	-	171	WP_001352098.1	conserved protein, Rac prophage	-
IB283_RS10010	2156279..2156500	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2150271..2199028	48757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T172179 WP_001317028.1 NZ_CP061185:c2152198-2152004 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T172179 NZ_CP079636:4136953-4137171 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT172179 NZ_CP061185:2151827-2152005 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGAAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin