Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1155660..1156339 Replicon chromosome
Accession NZ_CP061185
Organism Escherichia coli strain LD67-1

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag IB283_RS05265 Protein ID WP_000057541.1
Coordinates 1155660..1155962 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag IB283_RS05270 Protein ID WP_000806442.1
Coordinates 1155998..1156339 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB283_RS05245 1150923..1152143 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
IB283_RS05250 1152361..1154013 + 1653 WP_077516164.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
IB283_RS05255 1154050..1154529 - 480 WP_000186627.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
IB283_RS05260 1154733..1155527 - 795 WP_000365164.1 TraB/GumN family protein -
IB283_RS05265 1155660..1155962 + 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IB283_RS05270 1155998..1156339 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
IB283_RS05275 1156397..1158901 - 2505 WP_001601790.1 copper-exporting P-type ATPase CopA -
IB283_RS05280 1159164..1160096 + 933 WP_000883035.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T172174 WP_000057541.1 NZ_CP061185:1155660-1155962 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T172174 NZ_CP079636:335828-336196 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT172174 WP_000806442.1 NZ_CP061185:1155998-1156339 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT172174 NZ_CP079636:335610-335831 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References