Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 931563..932257 Replicon chromosome
Accession NZ_CP061185
Organism Escherichia coli strain LD67-1

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag IB283_RS04215 Protein ID WP_001263491.1
Coordinates 931859..932257 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag IB283_RS04210 Protein ID WP_000554755.1
Coordinates 931563..931856 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB283_RS04185 926599..926859 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
IB283_RS04190 927045..927818 + 774 WP_022645219.1 C40 family peptidase -
IB283_RS04195 927916..929655 - 1740 WP_022645220.1 flagellar biosynthesis protein FlhA -
IB283_RS04200 929600..930385 + 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
IB283_RS04205 930456..931511 + 1056 WP_022645222.1 DNA polymerase IV -
IB283_RS04210 931563..931856 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
IB283_RS04215 931859..932257 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
IB283_RS04220 932267..932719 + 453 WP_023909048.1 GNAT family N-acetyltransferase -
IB283_RS04225 932910..934049 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
IB283_RS04230 934046..934660 + 615 WP_022645225.1 peptide chain release factor H -
IB283_RS04235 934722..935231 + 510 WP_001361775.1 hydrolase -
IB283_RS04240 935248..935529 - 282 WP_022645226.1 hypothetical protein -
IB283_RS04245 935538..936995 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T172172 WP_001263491.1 NZ_CP061185:931859-932257 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T172172 NZ_CP079635:235890-235992 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT172172 WP_000554755.1 NZ_CP061185:931563-931856 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT172172 NZ_CP079635:c235999-235854 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References