Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1849980..1850482 Replicon chromosome
Accession NZ_CP061108
Organism Escherichia coli strain 1EC187

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IBZ91_RS08745 Protein ID WP_000767829.1
Coordinates 1850228..1850482 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IBZ91_RS08740 Protein ID WP_001259255.1
Coordinates 1849980..1850231 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBZ91_RS08715 1845157..1846224 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IBZ91_RS08720 1846224..1847294 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
IBZ91_RS08725 1847291..1848595 - 1305 WP_001317939.1 histidinol dehydrogenase -
IBZ91_RS08730 1848601..1849500 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IBZ91_RS08735 1849646..1849696 - 51 WP_001364200.1 his operon leader peptide -
IBZ91_RS08740 1849980..1850231 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IBZ91_RS08745 1850228..1850482 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IBZ91_RS08750 1850565..1851389 + 825 WP_000754758.1 SDR family oxidoreductase -
IBZ91_RS08755 1851435..1852364 + 930 WP_000803351.1 LysR family transcriptional regulator -
IBZ91_RS08760 1852579..1852641 + 63 WP_010723108.1 membrane protein YoeI -
IBZ91_RS08765 1852631..1853989 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IBZ91_RS08770 1854168..1855226 + 1059 WP_000492346.1 transport protein YeeE -
IBZ91_RS08775 1855240..1855467 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T172125 WP_000767829.1 NZ_CP061108:1850228-1850482 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T172125 NZ_CP079606:5071203-5071305 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT172125 WP_001259255.1 NZ_CP061108:1849980-1850231 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT172125 NZ_CP079606:c5071312-5071167 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References