Detailed information of TA system TA172123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1115897..1116624
• Replicon: chromosome
• Accession: NZ_CP061108
• Organism: Escherichia coli strain 1EC187

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: IBZ91_RS05395
• Protein ID: WP_000547564.1
• Coordinates: 1115897..1116208 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IBZ91_RS05400
• Protein ID: WP_000126294.1
• Coordinates: 1116205..1116624 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBZ91_RS05365	1111039..1112748	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
IBZ91_RS05370	1112758..1113300	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
IBZ91_RS05375	1113300..1114067	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
IBZ91_RS05380	1114064..1114474	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
IBZ91_RS05385	1114467..1114937	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
IBZ91_RS05390	1114962..1115723	+	762	WP_001026446.1	hypothetical protein	-
IBZ91_RS05395	1115897..1116208	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
IBZ91_RS05400	1116205..1116624	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
IBZ91_RS05405	1116738..1118162	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
IBZ91_RS05410	1118171..1119628	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
IBZ91_RS05415	1119888..1120898	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
IBZ91_RS05420	1121047..1121574	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T172123 WP_000547564.1 NZ_CP061108:1115897-1116208 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T172123 NZ_CP079606:3394306-3394674 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT172123 WP_000126294.1 NZ_CP061108:1116205-1116624 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT172123 NZ_CP079606:3394088-3394309 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5