Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 645030..645829 Replicon chromosome
Accession NZ_CP061108
Organism Escherichia coli strain 1EC187

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag IBZ91_RS03095 Protein ID WP_000347251.1
Coordinates 645030..645494 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IBZ91_RS03100 Protein ID WP_001307405.1
Coordinates 645494..645829 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBZ91_RS03065 640031..640465 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IBZ91_RS03070 640483..641361 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IBZ91_RS03075 641351..642130 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IBZ91_RS03080 642141..642614 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IBZ91_RS03085 642637..643917 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
IBZ91_RS03090 644166..644975 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IBZ91_RS03095 645030..645494 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IBZ91_RS03100 645494..645829 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IBZ91_RS03105 645978..647549 - 1572 WP_001273761.1 galactarate dehydratase -
IBZ91_RS03110 647924..649258 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
IBZ91_RS03115 649274..650044 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T172119 WP_000347251.1 NZ_CP061108:c645494-645030 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T172119 NZ_CP079606:1755898-1756116 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT172119 WP_001307405.1 NZ_CP061108:c645829-645494 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172119 NZ_CP079606:1755497-1755871 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References