Detailed information of TA system TA172112

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68238..68659
• Replicon: plasmid pEC213_1-OXA-181
• Accession: NZ_CP061102
• Organism: Escherichia coli strain 1EC213

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: IB009_RS23255
• Protein ID: WP_001302184.1
• Coordinates: 68238..68396 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	68461..68659 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB009_RS23215	63438..64135	-	698	Protein_62	IS1-like element IS1A family transposase	-
IB009_RS23220	64192..64335	-	144	Protein_63	conjugal transfer protein TraJ	-
IB009_RS23225	64522..64905	-	384	WP_001151524.1	relaxosome protein TraM	-
IB009_RS23230	65238..65828	+	591	WP_175345273.1	transglycosylase SLT domain-containing protein	-
IB009_RS23235	66124..66945	-	822	WP_001234417.1	DUF945 domain-containing protein	-
IB009_RS23240	67064..67351	-	288	WP_000107542.1	hypothetical protein	-
IB009_RS23245	67326..67583	-	258	WP_199206704.1	single-stranded DNA-binding protein	-
IB009_RS23250	67653..68000	+	348	Protein_69	hypothetical protein	-
IB009_RS23255	68238..68396	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	68461..68659	-	199	NuclAT_0	-	Antitoxin
-	68461..68659	-	199	NuclAT_0	-	Antitoxin
-	68461..68659	-	199	NuclAT_0	-	Antitoxin
-	68461..68659	-	199	NuclAT_0	-	Antitoxin
IB009_RS23260	68671..69390	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
IB009_RS23265	69387..69821	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
IB009_RS23270	69876..71834	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
IB009_RS23275	71900..72133	-	234	WP_000005990.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaOXA-181 / qnrS1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-35	-	1..95832	95832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T172112 WP_001302184.1 NZ_CP061102:c68396-68238 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T172112 NZ_CP079599:4184206-4184490 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT172112 NZ_CP061102:c68659-68461 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin