Detailed information of TA system TA172109

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51215..51469
• Replicon: plasmid pEC213_1-OXA-181
• Accession: NZ_CP061102
• Organism: Escherichia coli strain 1EC213

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: IB009_RS23160
• Protein ID: WP_001362723.1
• Coordinates: 51215..51421 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	51408..51469 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IB009_RS23125	47288..47533	-	246	Protein_44	3'-5' exonuclease	-
IB009_RS23130	47579..47860	-	282	WP_000969980.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IB009_RS23135	47857..48126	-	270	WP_000079949.1	hypothetical protein	-
IB009_RS23140	49041..49898	-	858	WP_000774301.1	incFII family plasmid replication initiator RepA	-
IB009_RS23145	49891..50373	-	483	WP_001443034.1	hypothetical protein	-
IB009_RS23150	50366..50440	-	75	WP_001442103.1	RepA leader peptide Tap	-
IB009_RS23155	50674..50931	-	258	WP_000083834.1	replication regulatory protein RepA	-
IB009_RS23160	51215..51421	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	51408..51469	+	62	NuclAT_1	-	Antitoxin
-	51408..51469	+	62	NuclAT_1	-	Antitoxin
-	51408..51469	+	62	NuclAT_1	-	Antitoxin
-	51408..51469	+	62	NuclAT_1	-	Antitoxin
IB009_RS23165	51765..52076	+	312	WP_000802277.1	hypothetical protein	-
IB009_RS23170	52257..52343	-	87	Protein_53	endonuclease	-
IB009_RS23175	52541..52729	-	189	WP_001490801.1	hypothetical protein	-
IB009_RS23180	52864..53424	-	561	WP_000139310.1	fertility inhibition protein FinO	-
IB009_RS23185	53479..54225	-	747	WP_000205776.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaOXA-181 / qnrS1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-35	-	1..95832	95832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T172109 WP_001362723.1 NZ_CP061102:c51421-51215 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T172109 NZ_CP079599:c3276088-3275720 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT172109 NZ_CP061102:51408-51469 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin