Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4535314..4535916 Replicon chromosome
Accession NZ_CP061101
Organism Escherichia coli strain 1EC213

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag IB009_RS21890 Protein ID WP_000897305.1
Coordinates 4535605..4535916 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IB009_RS21885 Protein ID WP_000356397.1
Coordinates 4535314..4535604 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB009_RS21860 4531240..4532142 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
IB009_RS21865 4532139..4532774 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
IB009_RS21870 4532771..4533700 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
IB009_RS21875 4534030..4534272 - 243 WP_001086388.1 hypothetical protein -
IB009_RS21880 4534491..4534709 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
IB009_RS21885 4535314..4535604 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
IB009_RS21890 4535605..4535916 - 312 WP_000897305.1 hypothetical protein Toxin
IB009_RS21895 4536145..4537053 + 909 WP_001162704.1 alpha/beta hydrolase -
IB009_RS21900 4537117..4538058 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
IB009_RS21905 4538103..4538540 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
IB009_RS21910 4538537..4539409 - 873 WP_000920762.1 virulence factor BrkB family protein -
IB009_RS21915 4539403..4540002 - 600 WP_086732335.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T172107 WP_000897305.1 NZ_CP061101:c4535916-4535605 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T172107 NZ_CP079599:c1621884-1621782 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT172107 WP_000356397.1 NZ_CP061101:c4535604-4535314 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT172107 NZ_CP079599:1621775-1621920 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References