Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3718002..3718543 Replicon chromosome
Accession NZ_CP061101
Organism Escherichia coli strain 1EC213

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag IB009_RS18135 Protein ID WP_000615976.1
Coordinates 3718265..3718543 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag IB009_RS18130 Protein ID WP_000729704.1
Coordinates 3718002..3718262 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB009_RS18105 3713785..3714570 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
IB009_RS18110 3714542..3716254 + 1713 Protein_3542 flagellar biosynthesis protein FlhA -
IB009_RS18115 3716359..3716637 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
IB009_RS18120 3716630..3716986 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
IB009_RS18125 3717043..3717816 - 774 WP_000543899.1 C40 family peptidase -
IB009_RS18130 3718002..3718262 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IB009_RS18135 3718265..3718543 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IB009_RS18140 3718699..3719439 + 741 WP_001225679.1 murein L,D-transpeptidase -
IB009_RS18145 3719410..3720177 - 768 WP_000333380.1 class II glutamine amidotransferase -
IB009_RS18150 3720383..3720961 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T172102 WP_000615976.1 NZ_CP061101:3718265-3718543 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T172102 NZ_CP079225:c5150295-5150041 [Rhodococcus sp. LW-XY12]
GTGAACATCACCTTCACCGACGAAGCGTGGGACGACTACCAGTTCTGGATCTCGACCGACAGAAAACAGCTCGAGCGTGT
CAACGAGCTGGTCAGGGATTGCCAACGAGACCCGTACTCGGGGATCGGCAAACCCGAACGGCTCAGACATGCCCTGTCAG
GACTGTGGTCTCGACGTATCGACAAAGAACACCGCCTGGTCTACGAGGTCATCGACGGTGCCCTGGTCATCATCGCCGCC
CGCTACCACTACTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT172102 WP_000729704.1 NZ_CP061101:3718002-3718262 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT172102 NZ_CP079225:c5150540-5150292 [Rhodococcus sp. LW-XY12]
ATGGCTGCCATCACTGCTACCGAAGCGAGAAAAAACTTCTTTCCGCTGCTCGAACAGGTCAACAAGGACCACACCCCTGT
GGAAATCGTATCCAAGCGGGGCAATGCCGTCCTGATGTCGAAGGATGACTACGACGCCATGCTCGAGACCTCGTACCTGA
TGCAGTCTCCGGCCAACGCCCGACGACTGCTCGCCAGCATGCAGAGAGCTCGCGGAGGTGAATACACCGAAAGGGAACTC
ATCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References