Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 620623..621422 Replicon chromosome
Accession NZ_CP061101
Organism Escherichia coli strain 1EC213

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag IB009_RS02980 Protein ID WP_000347266.1
Coordinates 620623..621087 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IB009_RS02985 Protein ID WP_001307405.1
Coordinates 621087..621422 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB009_RS02950 615624..616058 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IB009_RS02955 616076..616954 - 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IB009_RS02960 616944..617723 - 780 WP_162676424.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IB009_RS02965 617734..618207 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IB009_RS02970 618230..619510 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
IB009_RS02975 619759..620568 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IB009_RS02980 620623..621087 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IB009_RS02985 621087..621422 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IB009_RS02990 621571..623142 - 1572 WP_001273741.1 galactarate dehydratase -
IB009_RS02995 623517..624851 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IB009_RS03000 624867..625637 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 620623..632078 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T172083 WP_000347266.1 NZ_CP061101:c621087-620623 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T172083 NZ_CP079193:c1166964-1166197 [Streptococcus suis]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATTTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT172083 WP_001307405.1 NZ_CP061101:c621422-621087 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172083 NZ_CP079193:c1167440-1166964 [Streptococcus suis]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCATCTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCAAAGCTTTATAGATACCAGGATAGTCAAGATATTGTTTTTGATGATGA
ATCTAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATCAATACGAAAATTTACCTTGTCGCTACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGATGGTATAGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References