Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 610593..611183 Replicon chromosome
Accession NZ_CP061089
Organism Brucella sp. 6810

Toxin (Protein)


Gene name graT Uniprot ID A0A7L7MSL3
Locus tag IB024_RS14005 Protein ID WP_069714895.1
Coordinates 610593..610874 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A7L7MR57
Locus tag IB024_RS14010 Protein ID WP_008510700.1
Coordinates 610887..611183 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IB024_RS13990 606172..607509 + 1338 WP_187960517.1 DNA polymerase IV -
IB024_RS13995 607685..609874 + 2190 WP_187960518.1 esterase-like activity of phytase family protein -
IB024_RS14005 610593..610874 + 282 WP_069714895.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IB024_RS14010 610887..611183 + 297 WP_008510700.1 HigA family addiction module antidote protein Antitoxin
IB024_RS14015 611433..611615 + 183 WP_187960519.1 hypothetical protein -
IB024_RS14020 611748..612467 - 720 WP_187960520.1 GGDEF domain-containing protein -
IB024_RS14025 613312..614262 + 951 WP_187960521.1 IS481 family transposase -
IB024_RS14035 615222..615599 - 378 WP_187960522.1 pyrimidine dimer DNA glycosylase/endonuclease V -
IB024_RS14040 615740..616045 + 306 WP_002971236.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(16-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-68)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10569.00 Da        Isoelectric Point: 8.0374

>T172079 WP_069714895.1 NZ_CP061089:610593-610874 [Brucella sp. 6810]
MIRSYKNSVTETVAWGKAPKGFPSDLVRVSVRKLTMLDNAVTLDDLRSPPGNRLEALKGDRQGQHSIRINDQWRICFVWT
DAGAEQVEIVDYH

Download         Length: 282 bp

>T172079 NZ_CP079188:c44093-43677 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11001.76 Da        Isoelectric Point: 5.6882

>AT172079 WP_008510700.1 NZ_CP061089:610887-611183 [Brucella sp. 6810]
MTNILPPVHPGEILREEYLIPLGMSAGALAKRLNVPRTRIERLATEQTNVTPDTALRLAKFLRTTPEFWMNMQASYDLKT
EAVAIQDQLAAIHEMEAA

Download         Length: 297 bp

>AT172079 NZ_CP079188:c44320-44090 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L7MSL3

Antitoxin

Source ID Structure
AlphaFold DB A0A7L7MR57

References