Detailed information of TA system TA172067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4787399..4788129
• Replicon: chromosome
• Accession: NZ_CP061082
• Organism: Serratia liquefaciens strain MT49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A515D591
• Locus tag: IAI46_RS22495
• Protein ID: WP_041415720.1
• Coordinates: 4787399..4787710 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IAI46_RS22500
• Protein ID: WP_020831880.1
• Coordinates: 4787707..4788129 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI46_RS22465	4782684..4783409	+	726	WP_020831867.1	LPS export ABC transporter ATP-binding protein	-
IAI46_RS22470	4783460..4784893	+	1434	WP_020831868.1	RNA polymerase factor sigma-54	-
IAI46_RS22475	4784917..4785204	+	288	WP_004948547.1	ribosome hibernation promoting factor	-
IAI46_RS22480	4785363..4785824	+	462	WP_115060613.1	PTS IIA-like nitrogen regulatory protein PtsN	-
IAI46_RS22485	4785956..4786810	+	855	WP_012147065.1	RNase adapter RapZ	-
IAI46_RS22490	4786807..4787079	+	273	WP_020831874.1	PTS phosphocarrier protein NPr	-
IAI46_RS22495	4787399..4787710	+	312	WP_041415720.1	type II toxin-antitoxin system HigB family toxin	Toxin
IAI46_RS22500	4787707..4788129	+	423	WP_020831880.1	helix-turn-helix domain-containing protein	Antitoxin
IAI46_RS22505	4788146..4790062	-	1917	WP_020831882.1	BglG family transcription antiterminator	-
IAI46_RS22510	4790114..4791331	-	1218	WP_122080299.1	lactonase family protein	-
IAI46_RS22515	4791483..4792223	-	741	WP_044553680.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12299.20 Da
• Isoelectric Point: 10.4493

>T172067 WP_041415720.1 NZ_CP061082:4787399-4787710 [Serratia liquefaciens]
MHIISREPFNEAAIRYPNEAASLNTIYKTLRRGTFHTPEDLKSLFPSLDRMKYKAKWWVIDVGGNHLRILFFASFDTQKI
FVKQIVTHAEYDRLLQQYRRNPK

Nucleotide

• Length: 312 bp

>T172067 NZ_CP079181:c44093-43677 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15503.82 Da
• Isoelectric Point: 4.9381

>AT172067 WP_020831880.1 NZ_CP061082:4787707-4788129 [Serratia liquefaciens]
MITDALKATHELLRAIPLLGGSTSPQDYRDALALVEHLLETDERHPLIDVLAAKIVEYEDNSPDFAEFNQRIAQLPQGVA
ALSVLMEQYGLSQSDFENEIGKKSLVSQILSGKRSLTIPHIMALAKRFNLPPALFLPEAR

Nucleotide

• Length: 423 bp

>AT172067 NZ_CP079181:c44320-44090 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A515D591