Detailed information of TA system TA172022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 526590..527284
• Replicon: chromosome
• Accession: NZ_CP061079
• Organism: Pseudomonas chlororaphis strain qlu-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HLB40_RS02365
• Protein ID: WP_101281702.1
• Coordinates: 526590..526886 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A554PLA7
• Locus tag: HLB40_RS02370
• Protein ID: WP_009046631.1
• Coordinates: 526883..527284 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLB40_RS02330	522124..522669	-	546	WP_009046626.1	membrane protein	-
HLB40_RS02335	522666..522920	-	255	WP_007926835.1	acyl carrier protein	-
HLB40_RS02340	522930..523190	-	261	WP_007926834.1	acyl carrier protein	-
HLB40_RS02345	523165..523980	-	816	WP_016704468.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
HLB40_RS02350	523956..524687	-	732	WP_036985559.1	beta-ketoacyl synthase chain length factor	-
HLB40_RS02355	525021..525791	+	771	WP_007926828.1	ParA family protein	-
HLB40_RS02360	525917..526351	-	435	WP_016704469.1	thioredoxin TrxC	-
HLB40_RS02365	526590..526886	+	297	WP_101281702.1	type II toxin-antitoxin system HigB family toxin	Toxin
HLB40_RS02370	526883..527284	+	402	WP_009046631.1	transcriptional regulator	Antitoxin
HLB40_RS02375	527288..528751	-	1464	WP_016704471.1	YdiU family protein	-
HLB40_RS02380	528800..532150	-	3351	WP_009046633.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Antitoxin HigA (15-133)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11517.33 Da
• Isoelectric Point: 10.5069

>T172022 WP_101281702.1 NZ_CP061079:526590-526886 [Pseudomonas chlororaphis]
MHIITAKRIWEAKSRWPQSANALETWYRKVKSLNPADFAAIKAVFPATDKVGVFHVFDIGGNKLRIIAVVRYEVRRLYIR
HVLDHHEYDKGRWKEETG

Nucleotide

• Length: 297 bp

>T172022 NZ_CP079155:c1348439-1348029 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGAAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14840.85 Da
• Isoelectric Point: 4.4394

>AT172022 WP_009046631.1 NZ_CP061079:526883-527284 [Pseudomonas chlororaphis]
MNVLVKQAADHWHFVAPVLRKPNTEADYDALVAALDELLDLMGEDENHPVTSLVDIIGDWIEAYDEEHRPMPIASGVDVL
RSLMREHGLTQSDLPGVGAQSVVSEVLSGKRQLNLRQIRWLAERFGVSAEVFI

Nucleotide

• Length: 402 bp

>AT172022 NZ_CP079155:c1348686-1348420 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A554PLA7