Detailed information of TA system TA172016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2211781..2212376
• Replicon: chromosome
• Accession: NZ_CP061073
• Organism: Pseudomonas aeruginosa strain PAD8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: IBG29_RS10370
• Protein ID: WP_003113526.1
• Coordinates: 2212098..2212376 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IBG29_RS10365
• Protein ID: WP_003099268.1
• Coordinates: 2211781..2212086 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBG29_RS10350 (IBG29_23705)	2208511..2209200	-	690	WP_128725157.1	hypothetical protein	-
IBG29_RS10355 (IBG29_23700)	2209197..2210474	-	1278	WP_128725156.1	hypothetical protein	-
IBG29_RS10360 (IBG29_23695)	2210615..2211274	-	660	WP_128725155.1	hypothetical protein	-
IBG29_RS10365 (IBG29_23690)	2211781..2212086	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
IBG29_RS10370 (IBG29_23685)	2212098..2212376	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IBG29_RS10375 (IBG29_30820)	2212429..2212557	-	129	Protein_2046	integrase	-
IBG29_RS10380 (IBG29_23680)	2212705..2214933	+	2229	WP_003113525.1	TonB-dependent receptor	-
IBG29_RS10385 (IBG29_23675)	2215003..2215650	-	648	WP_003095021.1	carbonate dehydratase	-
IBG29_RS10390 (IBG29_23670)	2215712..2216950	-	1239	WP_003123433.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T172016 WP_003113526.1 NZ_CP061073:c2212376-2212098 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T172016 NZ_CP079150:c5364010-5363627 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT172016 WP_003099268.1 NZ_CP061073:c2212086-2211781 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT172016 NZ_CP079150:c5364252-5364010 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: V6ALY3