Detailed information of TA system TA171954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5484954..5485549
• Replicon: chromosome
• Accession: NZ_CP061034
• Organism: Pseudomonas aeruginosa strain PA3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: EBO27_RS25640
• Protein ID: WP_003117425.1
• Coordinates: 5485271..5485549 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBO27_RS25635
• Protein ID: WP_003113527.1
• Coordinates: 5484954..5485259 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBO27_RS25610	5480021..5480356	+	336	WP_187787318.1	hypothetical protein	-
EBO27_RS25615	5480403..5480576	+	174	WP_128531165.1	NUDIX domain-containing protein	-
EBO27_RS25620	5480893..5481474	-	582	WP_128531166.1	hypothetical protein	-
EBO27_RS25625	5481475..5481906	-	432	WP_128531167.1	response regulator	-
EBO27_RS25630	5481903..5483678	-	1776	WP_187787319.1	HAMP domain-containing histidine kinase	-
EBO27_RS25635	5484954..5485259	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
EBO27_RS25640	5485271..5485549	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBO27_RS25645	5485878..5488106	+	2229	WP_003099265.1	TonB-dependent receptor	-
EBO27_RS25650	5488176..5488823	-	648	WP_003095021.1	carbonate dehydratase	-
EBO27_RS25655	5488885..5490123	-	1239	WP_031685043.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T171954 WP_003117425.1 NZ_CP061034:c5485549-5485271 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T171954 NZ_CP079120:c508902-508684 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT171954 WP_003113527.1 NZ_CP061034:c5485259-5484954 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT171954 NZ_CP079120:c509303-508929 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5