Detailed information of TA system TA171927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4969..5554
• Replicon: plasmid unnamed2
• Accession: NZ_CP060996
• Organism: Acinetobacter baumannii strain CAb-65

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: IAG34_RS19115
• Protein ID: WP_000897309.1
• Coordinates: 5234..5554 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IAG34_RS19110
• Protein ID: WP_005086343.1
• Coordinates: 4969..5241 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAG34_RS19090 (IAG34_19080)	126..1007	+	882	WP_005804946.1	replication initiation protein RepM	-
IAG34_RS19095 (IAG34_19085)	1071..1637	+	567	WP_139845804.1	plasmid replication DNA-binding protein	-
IAG34_RS19100 (IAG34_19090)	1868..2719	-	852	WP_004870777.1	universal stress protein	-
IAG34_RS19105 (IAG34_19095)	2732..4219	-	1488	WP_004870779.1	SulP family inorganic anion transporter	-
IAG34_RS19110 (IAG34_19100)	4969..5241	-	273	WP_005086343.1	NadS family protein	Antitoxin
IAG34_RS19115 (IAG34_19105)	5234..5554	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAG34_RS19120 (IAG34_19110)	5782..6435	+	654	WP_005086345.1	hypothetical protein	-
IAG34_RS19125 (IAG34_19115)	6510..7121	-	612	WP_004740252.1	recombinase family protein	-
IAG34_RS19130 (IAG34_19120)	7302..7832	-	531	Protein_8	plasmid pRiA4b ORF-3 family protein	-
IAG34_RS19135 (IAG34_19125)	7927..8241	-	315	WP_005086327.1	BrnA antitoxin family protein	-
IAG34_RS19140 (IAG34_19130)	8204..8515	-	312	WP_005086328.1	BrnT family toxin	-
IAG34_RS19430	8759..8831	-	73	Protein_11	hypothetical protein	-
IAG34_RS19435	8865..8957	-	93	WP_267460265.1	hypothetical protein	-
IAG34_RS19380	8954..9274	-	321	WP_228732867.1	hypothetical protein	-
IAG34_RS19150 (IAG34_19140)	9366..9805	-	440	Protein_14	DUF488 domain-containing protein	-
IAG34_RS19385	10110..10445	-	336	WP_228732868.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..13325	13325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T171927 WP_000897309.1 NZ_CP060996:c5554-5234 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T171927 NZ_CP079095:c1844300-1843005 [Methylococcus sp. Mc7]
ATGGCGCATGAGCTGGAAGTCTGGCTTTTCACCGATCGTGTCGGCACGTTGGCCCTGGTCGATGGACGACTAAACTTTTG
CTACGCACCCGATTGGCTGTCACACAAAGACGCCGTTGCCTTGTCCGCCTCGCTGCCCTTGCAAGCGGAGCCATTCGACG
ATCGCAAAGCGCGTCCTTTCTTTGCGGGTCTGCTGCCAGAGGGGCAGATGCGCCGCCTGATTGCGCAGCAGTTCCAGGTT
TCGGGCCAGAACGACTTTGCGCTGCTGGACCATATCGGCGGCGAATGCGCCGGAGCCGTGACGTTCCTTGAACCGGGGCA
GGCTTTGCCTGTGCCGACCCGCAACGATGACGTTCAATGGCTGAGCGACGACGAAGTCGTTGCCATCCTGGATGAATTGC
CGCGTCGCCCCATGTTGGCAGGCAAAGACGGCTTGCGACTTTCCTTGGCTGGTGCCCAAGACAAGTTGCCAGTGGTTTTC
GATGGTGCCCGCATTGGGCTGCCTCTCAACGGCACGCCGAGTTCCCACATCTTGAAACCCGCCATTCACGCTGTTGAAGA
CAGCGTGATCAACGAAGGGTTTTGCATGGCACTGGCTGACGCCATGCAGCTCAAGCCAGCGAAATCAAAAGTTCACCAGG
TATTGGATCGCTCGTTCCTGCTGGTTGAACGCTACGACCGACTGATCGATGCCCAGGGGCACCGGCAACGCCTTCATCAA
GAGGATTTCTGCCAGGCGATGGGCGTAGTGCCTGAAATGAAATACCAGAATGAAGGTGGTCCGGATCTGGCTCAGTGCTT
TGACCTGGTACGCAGTGCGACACGCCCCAGTGCACCGCAGGTTCTGCGACTGCTCGACTACGTAATCTTCAATGCGCTGA
TCGGCAATCATGATGCGCACGCCAAGAATTTCTCTCTGCTGTACGCAGGCAAGGCCCCCGTTCTGGCACCGTTTTACGAC
ACGCTCTCGACAGCGGTGTATCCAGCAATGACACCGAAGATGGCGATGAAAATCGGCAGCAAGTACAAGTTCAGTGAAGT
CCAGGCGCGGCACTGGGAGCAGTTTGCCGAAGGCGTAGGCTTTACCAAGGCTCAGGCCAAAAGGCGCATTCTGGAATTGG
CAAAGTCACTACCAGCCACTGCGCGCAAGCTCCAGTCTGCCCCTGAACGAGGCTTTGCCGGGAATGCCGTAGTTGAGCGA
ATCACCGCACTGATTGAACAACGCTGCACCCTGACGATTCGGCGGCTCACCGACCCTGCCGCCGAAAACGACGCACCCGA
CGAACCCTCTGTCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10094.53 Da
• Isoelectric Point: 7.2045

>AT171927 WP_005086343.1 NZ_CP060996:c5241-4969 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT171927 NZ_CP079095:c1844550-1844290 [Methylococcus sp. Mc7]
ATGACATCCATTCGATCACCTCAACAACTCGGCGATGCACTGCGTGCCGCTCGCAAGCAGCTCGGGCTGACTCAGCCTCA
GTTGGCGCTGGCGGCAGGGGTTGGTGTGCGCTTCATTGTCGACCTTGAATCAGGCAAGCCCACCTTGCGACTGGAAAACG
TGCTGCGGGTCATTGATGCCCTGGGTGGTGAGGTCCAGTTGAGTGGATTGCCATCGGTTGCTGCCGACGAACAACGTGAG
GGAGGCGACCATGGCGCATGA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3