Detailed information of TA system TA171925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 46904..47555
• Replicon: plasmid unnamed1
• Accession: NZ_CP060995
• Organism: Acinetobacter baumannii strain CAb-65

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0E2G754
• Locus tag: IAG34_RS18820
• Protein ID: WP_000269901.1
• Coordinates: 46904..47260 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IAG34_RS18825
• Protein ID: WP_001140619.1
• Coordinates: 47253..47555 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAG34_RS18805 (IAG34_18795)	43158..43664	-	507	WP_228732866.1	SMI1/KNR4 family protein	-
IAG34_RS18810 (IAG34_18800)	44348..44866	+	519	WP_000447197.1	tetratricopeptide repeat protein	-
IAG34_RS18815 (IAG34_18805)	45022..46352	+	1331	Protein_52	ISNCY family transposase	-
IAG34_RS18820 (IAG34_18810)	46904..47260	+	357	WP_000269901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAG34_RS18825 (IAG34_18815)	47253..47555	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
IAG34_RS18830 (IAG34_18820)	47548..47775	+	228	WP_217408638.1	hypothetical protein	-
IAG34_RS18835 (IAG34_18825)	47864..48091	-	228	WP_000286812.1	hypothetical protein	-
IAG34_RS18840 (IAG34_18830)	48848..49393	-	546	Protein_57	IS5-like element ISAba13 family transposase	-
IAG34_RS18845 (IAG34_18835)	49321..49710	-	390	WP_002072923.1	AAA family ATPase	-
IAG34_RS18850 (IAG34_18840)	49898..50218	+	321	WP_000088946.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IAG34_RS18855 (IAG34_18845)	50516..50683	+	168	WP_002072926.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IAG34_RS18860 (IAG34_18850)	50670..50957	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-
IAG34_RS18865 (IAG34_18855)	51036..51290	-	255	WP_000236586.1	hypothetical protein	-
IAG34_RS18870 (IAG34_18860)	51487..52044	-	558	Protein_63	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91773	91773
	-	inside	IScluster/Tn	-	-	41670..49273	7603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13487.44 Da
• Isoelectric Point: 6.4631

>T171925 WP_000269901.1 NZ_CP060995:46904-47260 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T171925 NZ_CP079095:c1623915-1623604 [Methylococcus sp. Mc7]
ATGCCGCGCTTCGTCCTCACGAACAAAGCCAAGGCTGACTTGAAGTCCATCGGACGCCATACCGCCGATACCTGGGGACG
CGAACAGCGAAACCGCTATCTCGCATCGCTGGACGGCGCTTTCCACGAACCGGCGGCTGATCCCTTCAAAGGCGGGGACT
GCGGAGCCGTTCGCCCCGGCTACCGCAAGCTTAGAGTCGGCCGGCACATTGTCTTCTACCGCTTGCTTAACGCCGGCTGC
ATCGAGATCGTACGCGTCCTGCATGAACGCATGGACGTGGAATGCCATCTGCAGGGACCGGATACGGATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT171925 WP_001140619.1 NZ_CP060995:47253-47555 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT171925 NZ_CP079095:c1624157-1623915 [Methylococcus sp. Mc7]
ATGCACAAGAACACCAGCGTCACCTTGGGCCAGCATTTTGATCAGTTCATCGCCCAGCAGATCACCAAAGGGCGTTTTGC
TTCCGCGAGCGAAGTCATCCGGGCAGGACTGAGGGTGCTCGAAGAGAGGGAAACACGGCTCGAAGCTTTGGCGCGCGCCC
TGAAGGATGGGGAGGATAGCGGAAGGGCGGATTATTCCCTTGAAGGCCTGATCGAGGAGCTCGACCGGGAAAACGTTTCC
TGA

Structures

Toxin

• AlphaFold DB: A0A0E2G754