Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 59536..59800 Replicon plasmid pEC31-3
Accession NZ_CP060989
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag H9212_RS25370 Protein ID WP_001331364.1
Coordinates 59648..59800 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 59536..59593 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9212_RS25355 (54775) 54775..57066 - 2292 WP_001289272.1 F-type conjugative transfer protein TrbC -
H9212_RS25360 (57059) 57059..58129 - 1071 WP_001535705.1 IncI1-type conjugal transfer protein TrbB -
H9212_RS25365 (58148) 58148..59356 - 1209 WP_001535704.1 IncI1-type conjugal transfer protein TrbA -
- (59536) 59536..59593 - 58 NuclAT_0 - Antitoxin
- (59536) 59536..59593 - 58 NuclAT_0 - Antitoxin
- (59536) 59536..59593 - 58 NuclAT_0 - Antitoxin
- (59536) 59536..59593 - 58 NuclAT_0 - Antitoxin
H9212_RS25370 (59648) 59648..59800 + 153 WP_001331364.1 Hok/Gef family protein Toxin
H9212_RS25375 (59872) 59872..60123 - 252 WP_001535703.1 hypothetical protein -
H9212_RS26200 (60624) 60624..60719 + 96 WP_000609148.1 DinQ-like type I toxin DqlB -
H9212_RS25385 (60784) 60784..60960 - 177 WP_001054904.1 hypothetical protein -
H9212_RS25390 (61169) 61169..61378 - 210 WP_001140545.1 hemolysin expression modulator Hha -
H9212_RS25395 (61476) 61476..62090 - 615 WP_000578649.1 plasmid IncI1-type surface exclusion protein ExcA -
H9212_RS25400 (62166) 62166..64334 - 2169 WP_000698360.1 IncI1-type conjugal transfer membrane protein TraY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / blaCTX-M-90 / floR / sul2 - 1..105686 105686


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T171919 WP_001331364.1 NZ_CP060989:59648-59800 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T171919 NZ_CP078521:c2444571-2444464 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 58 bp

>AT171919 NZ_CP060989:c59593-59536 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References