Detailed information of TA system TA171916

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111451..111774
• Replicon: plasmid pEC31-2
• Accession: NZ_CP060988
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H9212_RS24840
• Protein ID: WP_001372321.1
• Coordinates: 111451..111576 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	111653..111774 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9212_RS24805 (106950)	106950..107552	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
H9212_RS24810 (107849)	107849..108670	-	822	WP_001234469.1	DUF932 domain-containing protein	-
H9212_RS24815 (108962)	108962..110158	+	1197	WP_039023277.1	IS110-like element ISEc20 family transposase	-
H9212_RS24820 (110242)	110242..110529	-	288	WP_000107535.1	hypothetical protein	-
H9212_RS24825 (110554)	110554..110760	-	207	WP_000547939.1	hypothetical protein	-
H9212_RS24830 (110830)	110830..111003	+	174	Protein_120	hypothetical protein	-
H9212_RS24835 (111001)	111001..111231	-	231	WP_071587244.1	hypothetical protein	-
H9212_RS24840 (111451)	111451..111576	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H9212_RS24845 (111518)	111518..111667	-	150	Protein_123	plasmid maintenance protein Mok	-
- (111653)	111653..111774	-	122	NuclAT_0	-	Antitoxin
- (111653)	111653..111774	-	122	NuclAT_0	-	Antitoxin
- (111653)	111653..111774	-	122	NuclAT_0	-	Antitoxin
- (111653)	111653..111774	-	122	NuclAT_0	-	Antitoxin
H9212_RS24850 (111860)	111860..112606	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
H9212_RS24855 (112621)	112621..114162	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
- (114355)	114355..114463	-	109	NuclAT_1	-	-
- (114355)	114355..114463	-	109	NuclAT_1	-	-
- (114355)	114355..114463	-	109	NuclAT_1	-	-
- (114355)	114355..114463	-	109	NuclAT_1	-	-
H9212_RS24860 (114432)	114432..115194	-	763	Protein_126	plasmid SOS inhibition protein A	-
H9212_RS24865 (115191)	115191..115625	-	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / qnrS1 / dfrA14 / fosA3 / blaCTX-M-90 / sitABCD	-	1..141542	141542
	-	inside	IScluster/Tn	-	-	108962..114162	5200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T171916 WP_001372321.1 NZ_CP060988:c111576-111451 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T171916 NZ_CP078521:c2101737-2101528 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Nucleotide

• Length: 122 bp

>AT171916 NZ_CP060988:c111774-111653 [Escherichia coli]
AGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAAGTTAAT
TTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin