171910

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3910314..3911008 Replicon chromosome
Accession NZ_CP060987
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H9212_RS19060 Protein ID WP_001263489.1
Coordinates 3910314..3910712 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H9212_RS19065 Protein ID WP_000554758.1
Coordinates 3910715..3911008 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3905902) 3905902..3905982 - 81 NuclAT_13 - -
- (3905902) 3905902..3905982 - 81 NuclAT_13 - -
- (3905902) 3905902..3905982 - 81 NuclAT_13 - -
- (3905902) 3905902..3905982 - 81 NuclAT_13 - -
H9212_RS19035 (3906578) 3906578..3907036 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H9212_RS19040 (3907297) 3907297..3908754 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
H9212_RS19045 (3908811) 3908811..3909332 - 522 Protein_3754 peptide chain release factor H -
H9212_RS19050 (3909328) 3909328..3909534 - 207 Protein_3755 RtcB family protein -
H9212_RS19055 (3909852) 3909852..3910304 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
H9212_RS19060 (3910314) 3910314..3910712 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H9212_RS19065 (3910715) 3910715..3911008 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H9212_RS19070 (3911060) 3911060..3912115 - 1056 WP_001226164.1 DNA polymerase IV -
H9212_RS19075 (3912186) 3912186..3912971 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9212_RS19080 (3912943) 3912943..3914655 + 1713 Protein_3761 flagellar biosynthesis protein FlhA -
H9212_RS19085 (3914879) 3914879..3915376 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T171910 WP_001263489.1 NZ_CP060987:c3910712-3910314 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T171910 NZ_CP078521:c804970-804782 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT171910 WP_000554758.1 NZ_CP060987:c3911008-3910715 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT171910 NZ_CP078521:c805259-804999 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References