Detailed information of TA system TA171875

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76659..76913
• Replicon: plasmid pEC3-2
• Accession: NZ_CP060980
• Organism: Escherichia coli strain EC3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H9191_RS23265
• Protein ID: WP_001312851.1
• Coordinates: 76659..76808 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	76852..76913 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9191_RS23225 (72211)	72211..72612	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H9191_RS23230 (72545)	72545..72802	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H9191_RS23235 (72895)	72895..73548	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H9191_RS23240 (74487)	74487..75344	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H9191_RS23245 (75337)	75337..75819	-	483	WP_001273588.1	hypothetical protein	-
H9191_RS23250 (75812)	75812..75859	-	48	WP_229471593.1	hypothetical protein	-
H9191_RS23255 (75850)	75850..76101	+	252	WP_223195197.1	replication protein RepA	-
H9191_RS23260 (76118)	76118..76375	-	258	WP_000083845.1	replication regulatory protein RepA	-
H9191_RS23265 (76659)	76659..76808	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (76852)	76852..76913	+	62	NuclAT_1	-	Antitoxin
- (76852)	76852..76913	+	62	NuclAT_1	-	Antitoxin
- (76852)	76852..76913	+	62	NuclAT_1	-	Antitoxin
- (76852)	76852..76913	+	62	NuclAT_1	-	Antitoxin
H9191_RS23270 (77169)	77169..77243	-	75	Protein_80	endonuclease	-
H9191_RS23275 (77489)	77489..77701	-	213	WP_001299730.1	ANR family transcriptional regulator	-
H9191_RS23280 (77837)	77837..78397	-	561	WP_000139315.1	fertility inhibition protein FinO	-
H9191_RS23285 (78500)	78500..79360	-	861	WP_000704514.1	alpha/beta hydrolase	-
H9191_RS23290 (79419)	79419..80165	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C / dfrA14 / qnrS1 / tet(A) / floR / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..140376	140376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T171875 WP_001312851.1 NZ_CP060980:c76808-76659 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T171875 NZ_CP078146:c3563342-3563010 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT171875 NZ_CP060980:76852-76913 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin