Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3654630..3655171 Replicon chromosome
Accession NZ_CP060979
Organism Escherichia coli strain EC3

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9191_RS18015 Protein ID WP_000615983.1
Coordinates 3654893..3655171 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9191_RS18010 Protein ID WP_000729703.1
Coordinates 3654630..3654890 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9191_RS17990 (3650305) 3650305..3651090 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9191_RS17995 (3651062) 3651062..3652774 + 1713 Protein_3539 flagellar biosynthesis protein FlhA -
H9191_RS18000 (3652998) 3652998..3653495 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9191_RS18005 (3653671) 3653671..3654420 - 750 WP_000056849.1 C40 family peptidase -
H9191_RS18010 (3654630) 3654630..3654890 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9191_RS18015 (3654893) 3654893..3655171 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9191_RS18020 (3655327) 3655327..3656067 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9191_RS18025 (3656038) 3656038..3656805 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9191_RS18030 (3657011) 3657011..3657589 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T171872 WP_000615983.1 NZ_CP060979:3654893-3655171 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171872 NZ_CP078146:c1127111-1126893 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT171872 WP_000729703.1 NZ_CP060979:3654630-3654890 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171872 NZ_CP078146:c1127512-1127138 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References