Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1794047..1794549 Replicon chromosome
Accession NZ_CP060979
Organism Escherichia coli strain EC3

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag H9191_RS08655 Protein ID WP_000767831.1
Coordinates 1794295..1794549 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H9191_RS08650 Protein ID WP_001259255.1
Coordinates 1794047..1794298 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9191_RS08625 (1789224) 1789224..1790291 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H9191_RS08630 (1790291) 1790291..1791361 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
H9191_RS08635 (1791358) 1791358..1792662 - 1305 WP_001565639.1 histidinol dehydrogenase -
H9191_RS08640 (1792668) 1792668..1793567 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H9191_RS08645 (1793713) 1793713..1793763 - 51 WP_001364200.1 his operon leader peptide -
H9191_RS08650 (1794047) 1794047..1794298 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H9191_RS08655 (1794295) 1794295..1794549 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H9191_RS08660 (1794632) 1794632..1795456 + 825 WP_000754737.1 SDR family oxidoreductase -
H9191_RS08665 (1795502) 1795502..1796431 + 930 WP_032258477.1 LysR substrate-binding domain-containing protein -
H9191_RS08670 (1796646) 1796646..1796708 + 63 WP_010723108.1 membrane protein YoeI -
H9191_RS08675 (1796698) 1796698..1798056 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H9191_RS08680 (1798235) 1798235..1799293 + 1059 WP_032258476.1 thiosulfate utilization transporter TsuA/YeeE -
H9191_RS08685 (1799307) 1799307..1799534 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T171857 WP_000767831.1 NZ_CP060979:1794295-1794549 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T171857 NZ_CP078141:18245-18523 [Pseudomonas savastanoi pv. savastanoi]
ATGCAAATCATCTGGCGGCAACGTGCGCGCATGAGCCTGGCTAAAATCATCCGGTATATCTCCAACGAAGACCCGCAAGC
CGCCCAAGCGATTCTAGAGCGTTTACAGTCGGCCATACTGCCGGTGGCCGATCATCCATACCTGTACCGCCCTGGACGTG
TGCCAGGCACCCGCGAGCTGGTGGCCCACCCGAACTACGTCCTGGTCTACCGCGTCACGCTTGAGCGCATCGAGGTCGTG
AACGTAATCCACGCTAGGCAGGAATACCCCTCGCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT171857 WP_001259255.1 NZ_CP060979:1794047-1794298 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT171857 NZ_CP078141:18045-18245 [Pseudomonas savastanoi pv. savastanoi]
ATGGCCAACCTCTCACCCATCGTTTCGGAATTCGAAACCGATGAACAAGCAGCAAGCTACGACCGTTGGTTTCGGCTCCA
GGTGCAAGCATCACTGGACGATCCCAGTCCAGGTGTGCCGCACGATCAGGTCATGGCGGAAATGGACGCAATCATTGCCG
AGGCAGAAAAGCGTCAACAGGATCGGACTAAGGTCAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References