Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3784405..3784946 Replicon chromosome
Accession NZ_CP060974
Organism Escherichia coli strain EC4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9192_RS18475 Protein ID WP_000615983.1
Coordinates 3784668..3784946 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9192_RS18470 Protein ID WP_000729703.1
Coordinates 3784405..3784665 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9192_RS18450 (3780080) 3780080..3780865 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9192_RS18455 (3780837) 3780837..3782549 + 1713 Protein_3630 flagellar biosynthesis protein FlhA -
H9192_RS18460 (3782773) 3782773..3783270 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9192_RS18465 (3783446) 3783446..3784195 - 750 WP_000056849.1 C40 family peptidase -
H9192_RS18470 (3784405) 3784405..3784665 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9192_RS18475 (3784668) 3784668..3784946 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9192_RS18480 (3785102) 3785102..3785842 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9192_RS18485 (3785813) 3785813..3786580 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9192_RS18490 (3786786) 3786786..3787364 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / rhs/PAAR / vgrG/tssI 3706037..3799915 93878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T171837 WP_000615983.1 NZ_CP060974:3784668-3784946 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171837 NZ_CP078137:c1853874-1853698 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT171837 WP_000729703.1 NZ_CP060974:3784405-3784665 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171837 NZ_CP078137:c1853652-1853236 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References