Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3778208..3778902 Replicon chromosome
Accession NZ_CP060974
Organism Escherichia coli strain EC4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H9192_RS18435 Protein ID WP_001263489.1
Coordinates 3778208..3778606 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H9192_RS18440 Protein ID WP_000554758.1
Coordinates 3778609..3778902 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3773796) 3773796..3773876 - 81 NuclAT_11 - -
- (3773796) 3773796..3773876 - 81 NuclAT_11 - -
- (3773796) 3773796..3773876 - 81 NuclAT_11 - -
- (3773796) 3773796..3773876 - 81 NuclAT_11 - -
H9192_RS18410 (3774472) 3774472..3774930 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H9192_RS18415 (3775191) 3775191..3776648 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
H9192_RS18420 (3776705) 3776705..3777226 - 522 Protein_3623 peptide chain release factor H -
H9192_RS18425 (3777222) 3777222..3777428 - 207 Protein_3624 RtcB family protein -
H9192_RS18430 (3777746) 3777746..3778198 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
H9192_RS18435 (3778208) 3778208..3778606 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H9192_RS18440 (3778609) 3778609..3778902 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H9192_RS18445 (3778954) 3778954..3780009 - 1056 WP_001226164.1 DNA polymerase IV -
H9192_RS18450 (3780080) 3780080..3780865 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9192_RS18455 (3780837) 3780837..3782549 + 1713 Protein_3630 flagellar biosynthesis protein FlhA -
H9192_RS18460 (3782773) 3782773..3783270 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / rhs/PAAR / vgrG/tssI 3706037..3799915 93878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T171836 WP_001263489.1 NZ_CP060974:c3778606-3778208 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T171836 NZ_CP078137:1748631-1749953 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT171836 WP_000554758.1 NZ_CP060974:c3778902-3778609 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT171836 NZ_CP078137:1748365-1748631 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References