Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1865662..1866164 Replicon chromosome
Accession NZ_CP060974
Organism Escherichia coli strain EC4

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag H9192_RS08840 Protein ID WP_000767831.1
Coordinates 1865910..1866164 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H9192_RS08835 Protein ID WP_001259255.1
Coordinates 1865662..1865913 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9192_RS08810 (1860839) 1860839..1861906 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H9192_RS08815 (1861906) 1861906..1862976 - 1071 WP_000108939.1 histidinol-phosphate transaminase -
H9192_RS08820 (1862973) 1862973..1864277 - 1305 WP_001375298.1 histidinol dehydrogenase -
H9192_RS08825 (1864283) 1864283..1865182 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H9192_RS08830 (1865328) 1865328..1865378 - 51 WP_001364200.1 his operon leader peptide -
H9192_RS08835 (1865662) 1865662..1865913 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H9192_RS08840 (1865910) 1865910..1866164 + 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H9192_RS08845 (1866247) 1866247..1867071 + 825 WP_000754737.1 SDR family oxidoreductase -
H9192_RS08850 (1867117) 1867117..1868046 + 930 WP_048217808.1 LysR substrate-binding domain-containing protein -
H9192_RS08855 (1868261) 1868261..1868323 + 63 WP_010723108.1 membrane protein YoeI -
H9192_RS08860 (1868313) 1868313..1869671 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H9192_RS08865 (1869850) 1869850..1870908 + 1059 WP_048217810.1 thiosulfate utilization transporter TsuA/YeeE -
H9192_RS08870 (1870922) 1870922..1871149 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T171822 WP_000767831.1 NZ_CP060974:1865910-1866164 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T171822 NZ_CP078127:c10141-9827 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT171822 WP_001259255.1 NZ_CP060974:1865662-1865913 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT171822 NZ_CP078127:c10407-10129 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References