Detailed information of TA system TA171815

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110376..110629
• Replicon: plasmid pEC5-3
• Accession: NZ_CP060971
• Organism: Escherichia coli strain EC5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H9193_RS24760
• Protein ID: WP_001312851.1
• Coordinates: 110480..110629 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	110376..110435 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9193_RS24735 (107103)	107103..107849	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H9193_RS24740 (107904)	107904..108464	+	561	WP_000139328.1	fertility inhibition protein FinO	-
H9193_RS24745 (108596)	108596..108796	+	201	WP_015059022.1	hypothetical protein	-
H9193_RS24750 (109182)	109182..109781	+	600	WP_032083981.1	PIN domain-containing protein	-
H9193_RS24755 (109843)	109843..110175	+	333	WP_152916585.1	hypothetical protein	-
- (110376)	110376..110435	-	60	NuclAT_1	-	Antitoxin
- (110376)	110376..110435	-	60	NuclAT_1	-	Antitoxin
- (110376)	110376..110435	-	60	NuclAT_1	-	Antitoxin
- (110376)	110376..110435	-	60	NuclAT_1	-	Antitoxin
H9193_RS24760 (110480)	110480..110629	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
H9193_RS24765 (110913)	110913..111161	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..111472	111472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T171815 WP_001312851.1 NZ_CP060971:110480-110629 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T171815 NZ_CP078122:c5448814-5448431 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT171815 NZ_CP060971:c110435-110376 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin