Detailed information of TA system TA171812

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68349..68618
• Replicon: plasmid pEC5-3
• Accession: NZ_CP060971
• Organism: Escherichia coli strain EC5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H9193_RS24515
• Protein ID: WP_001372321.1
• Coordinates: 68493..68618 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	68349..68414 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9193_RS24480	64059..64586	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H9193_RS24485	64644..64877	+	234	WP_000006003.1	DUF905 family protein	-
H9193_RS24490	64938..66961	+	2024	Protein_78	ParB/RepB/Spo0J family partition protein	-
H9193_RS24495	67030..67464	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H9193_RS24500	67461..68223	+	763	Protein_80	plasmid SOS inhibition protein A	-
-	68192..68416	+	225	NuclAT_0	-	-
-	68192..68416	+	225	NuclAT_0	-	-
-	68192..68416	+	225	NuclAT_0	-	-
-	68192..68416	+	225	NuclAT_0	-	-
H9193_RS24505	68201..68380	-	180	WP_001309233.1	hypothetical protein	-
-	68349..68414	-	66	-	-	Antitoxin
H9193_RS24510	68402..68551	+	150	Protein_82	plasmid maintenance protein Mok	-
H9193_RS24515	68493..68618	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H9193_RS24520	68937..69233	-	297	Protein_84	hypothetical protein	-
H9193_RS24525	69533..69829	+	297	WP_001272251.1	hypothetical protein	-
H9193_RS24530	69940..70761	+	822	WP_001234445.1	DUF932 domain-containing protein	-
H9193_RS24535	71058..71705	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
H9193_RS24540	71982..72365	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
H9193_RS24545	72556..73242	+	687	WP_015059009.1	PAS domain-containing protein	-
H9193_RS24550	73336..73563	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..111472	111472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T171812 WP_001372321.1 NZ_CP060971:68493-68618 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T171812 NZ_CP078122:c4865436-4864903 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT171812 NZ_CP060971:c68414-68349 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin