Detailed information of TA system TA171804

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100503..100757
• Replicon: plasmid pEC5-2
• Accession: NZ_CP060970
• Organism: Escherichia coli strain EC5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H9193_RS23775
• Protein ID: WP_001312851.1
• Coordinates: 100608..100757 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	100503..100564 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9193_RS23750 (97251)	97251..97997	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
H9193_RS23755 (98056)	98056..98916	+	861	WP_000704514.1	alpha/beta hydrolase	-
H9193_RS23760 (99019)	99019..99579	+	561	WP_000139315.1	fertility inhibition protein FinO	-
H9193_RS23765 (99715)	99715..99927	+	213	WP_001299730.1	ANR family transcriptional regulator	-
H9193_RS23770 (100173)	100173..100247	+	75	Protein_102	endonuclease	-
- (100503)	100503..100564	-	62	NuclAT_2	-	Antitoxin
- (100503)	100503..100564	-	62	NuclAT_2	-	Antitoxin
- (100503)	100503..100564	-	62	NuclAT_2	-	Antitoxin
- (100503)	100503..100564	-	62	NuclAT_2	-	Antitoxin
H9193_RS23775 (100608)	100608..100757	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
H9193_RS23780 (101041)	101041..101298	+	258	WP_000083845.1	replication regulatory protein RepA	-
H9193_RS23785 (101315)	101315..101566	-	252	WP_223195197.1	replication protein RepA	-
H9193_RS23790 (101557)	101557..101604	+	48	WP_229471593.1	hypothetical protein	-
H9193_RS23795 (101597)	101597..102079	+	483	WP_001273588.1	hypothetical protein	-
H9193_RS23800 (102072)	102072..102929	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H9193_RS23805 (103868)	103868..104521	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H9193_RS23810 (104614)	104614..104871	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H9193_RS23815 (104804)	104804..105205	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR / dfrA12 / aadA2 / qacE / mph(A) / aph(3')-Ia / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..147909	147909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T171804 WP_001312851.1 NZ_CP060970:100608-100757 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T171804 NZ_CP078120:3635353-3635817 [Halomonas sp. 18071143]
ATGAGCGAGGTCACCGCTTACCGGCTGGTGAAGCGCAAGTTTCAGGAGAGCGCGTTTGATGGCGAGGGCGCACGGCTTTA
CGGCGGGCGCTGGAACAGCCCAGGCAATGCCTGTGTGTATGTAGCCAGCTCAGAGTCGTTGGCACTGCTGGAAATCATGG
TGCATCTGGAGAGCTATCGGCTGCTCAACGCCTATGCGCTGCTGCGACTGACGCTGCCCAATCAGAGCATTTTGAGCATT
GGAGAGGAAGATTTACCCGAAAACTGGCGGGAAGCGCCAGCACCTGCGGAAACCGCCGACCTGGGCGATGGCTGGCTAGC
CTCCGGCCAGAGCCTGGCGCTGGCCCTGCCCAGCGTGGTGGTGCCGAGAGAGCTTAATTACATGCTCAACCCGGCCCACC
CCTTGTTCGAGCACGCCGTTGCCAGCGCCGAGGAACTGCCGTTTCAACCCGATCCACGGCTGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT171804 NZ_CP060970:c100564-100503 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin