Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3760589..3761130 Replicon chromosome
Accession NZ_CP060969
Organism Escherichia coli strain EC5

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag H9193_RS18330 Protein ID WP_000615979.1
Coordinates 3760852..3761130 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H9193_RS18325 Protein ID WP_000729704.1
Coordinates 3760589..3760849 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9193_RS18305 (3756264) 3756264..3757049 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
H9193_RS18310 (3757021) 3757021..3758733 + 1713 Protein_3603 flagellar biosynthesis protein FlhA -
H9193_RS18315 (3758957) 3758957..3759454 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9193_RS18320 (3759630) 3759630..3760403 - 774 WP_123297815.1 C40 family peptidase -
H9193_RS18325 (3760589) 3760589..3760849 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9193_RS18330 (3760852) 3760852..3761130 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9193_RS18335 (3761286) 3761286..3762026 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9193_RS18340 (3761997) 3761997..3762764 - 768 WP_000333379.1 class II glutamine amidotransferase -
H9193_RS18345 (3762970) 3762970..3763548 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T171799 WP_000615979.1 NZ_CP060969:3760852-3761130 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171799 NZ_CP078120:2821739-2821990 [Halomonas sp. 18071143]
ATGGATGCCATCAGCTACACCGCCGCTAGAACCAACCTAGCAAAAACCATGGAGCAGGTCTGTGAAGACCACTCTCCTGT
GATCATCACCCGCAATAAGGCGCAATCCGTGGTCATGATCTCTCTCGAAGACTATGAAGCACTGCAAGAGACAGCCTACC
TCCTGCGCGCGCCCAAAAACGCCCGCCGTTTACTGGAATCCGTTGCCGAGTTGGAGCAAGGGGGCGGTCAGGAAAAGGAG
CTTCTTGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT171799 WP_000729704.1 NZ_CP060969:3760589-3760849 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171799 NZ_CP078120:2821987-2822241 [Halomonas sp. 18071143]
ATGAAGCTCATCTTTTCCGAGAACGCCTGGGAAGACTATTTATACTGGCAGAAAACTGACAAAAAGATTCTCAACCGAAT
CAACAAGCTAGTCAAAGAAATCAAGCGAGAGCCATTTGAAGGAGTCGGCAAGCCAGAACCCCTTAAACACAGCCTCGCGG
GTTACTGGTCTCGGCGAATCAACGAAGAACACCGCATCGTTTACAAGGTCACGGACGACGCTTTGCTTGTTGCCCAACTG
CGGTATCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References