Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 639587..640386 Replicon chromosome
Accession NZ_CP060969
Organism Escherichia coli strain EC5

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag H9193_RS03095 Protein ID WP_000347251.1
Coordinates 639587..640051 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag H9193_RS03100 Protein ID WP_001308975.1
Coordinates 640051..640386 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9193_RS03065 (634588) 634588..635022 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9193_RS03070 (635040) 635040..635918 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
H9193_RS03075 (635908) 635908..636687 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9193_RS03080 (636698) 636698..637171 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9193_RS03085 (637194) 637194..638474 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9193_RS03090 (638723) 638723..639532 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9193_RS03095 (639587) 639587..640051 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9193_RS03100 (640051) 640051..640386 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9193_RS03105 (640535) 640535..642106 - 1572 WP_064221457.1 galactarate dehydratase -
H9193_RS03110 (642481) 642481..643815 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9193_RS03115 (643831) 643831..644601 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T171778 WP_000347251.1 NZ_CP060969:c640051-639587 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T171778 NZ_CP078114:c943432-943013 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT171778 WP_001308975.1 NZ_CP060969:c640386-640051 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171778 NZ_CP078114:c943668-943432 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References