Detailed information of TA system TA171773

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43030..43294
• Replicon: plasmid pEC7-3
• Accession: NZ_CP060965
• Organism: Escherichia coli strain EC7

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: H9194_RS24805
• Protein ID: WP_001387489.1
• Coordinates: 43142..43294 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43030..43090 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9194_RS24790 (39132)	39132..40202	-	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-
H9194_RS24795 (40221)	40221..41429	-	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
- (41609)	41609..41666	-	58	NuclAT_1	-	-
- (41609)	41609..41666	-	58	NuclAT_1	-	-
- (41609)	41609..41666	-	58	NuclAT_1	-	-
- (41609)	41609..41666	-	58	NuclAT_1	-	-
H9194_RS24800 (41736)	41736..42515	-	780	WP_275450201.1	protein FinQ	-
- (43030)	43030..43090	-	61	NuclAT_0	-	Antitoxin
- (43030)	43030..43090	-	61	NuclAT_0	-	Antitoxin
- (43030)	43030..43090	-	61	NuclAT_0	-	Antitoxin
- (43030)	43030..43090	-	61	NuclAT_0	-	Antitoxin
H9194_RS24805 (43142)	43142..43294	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
H9194_RS24810 (43366)	43366..43617	-	252	WP_001291964.1	hypothetical protein	-
H9194_RS25770 (44118)	44118..44213	+	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
H9194_RS24820 (44278)	44278..44454	-	177	WP_001054900.1	hypothetical protein	-
H9194_RS24825 (44846)	44846..45055	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H9194_RS24830 (45127)	45127..45789	-	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
H9194_RS24835 (45860)	45860..48028	-	2169	WP_015508354.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..89379	89379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T171773 WP_001387489.1 NZ_CP060965:43142-43294 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T171773 NZ_CP078111:13373-13891 [Cronobacter sakazakii]
ATGTTTTCAGAAGAAGCCGTATACGACTTCTCAGACTTCGACTGTGGCGAAGCATCTCTAAATGAGTTTCTTAAAAATCG
CCTGGCACAGCAACACAGCGGGCGTATATTGCGCGGTTATCTGCTTCTGACCAAAGACGCGATACCGAAAGTTAAAGGGT
TTTACACGCTGTCCGGGAGCTGTTTCGCAAGACAAACGCTCCCATCCAACACGCAGCAGCGGAAGATACCTTATTCTGAT
GCCCCCAGCGTTACGCTCGGACGTCTGGCGATAGACAAGAGCATTCAACGGCAAGGTAAAGGTGAAGAATTGGTGGTAGA
TGCCATGAAAGTGGTCTACCAGGCTTCTCGTGCCGTTGGTATCTACGCGCTGTTTGTAGATGCAAAAAATCCGGCCGCCA
GGCAGTTTTACCAGAATCTGGGGTTTATCCCTCTGAAGGGTGAAAACGCCAACTCGCTTTTCTATCCAACCAAAAGCATT
GAGGCTCTGTTTGAAGAGAAGCCGTCTGAAGACGAATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT171773 NZ_CP060965:c43090-43030 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin