Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3758134..3758675 Replicon chromosome
Accession NZ_CP060963
Organism Escherichia coli strain EC7

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9194_RS18635 Protein ID WP_000615983.1
Coordinates 3758397..3758675 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9194_RS18630 Protein ID WP_000729703.1
Coordinates 3758134..3758394 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9194_RS18610 (3753809) 3753809..3754594 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9194_RS18615 (3754566) 3754566..3756278 + 1713 Protein_3661 flagellar biosynthesis protein FlhA -
H9194_RS18620 (3756502) 3756502..3756999 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9194_RS18625 (3757175) 3757175..3757924 - 750 WP_000056849.1 C40 family peptidase -
H9194_RS18630 (3758134) 3758134..3758394 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9194_RS18635 (3758397) 3758397..3758675 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9194_RS18640 (3758831) 3758831..3759571 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9194_RS18645 (3759542) 3759542..3760309 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9194_RS18650 (3760515) 3760515..3761093 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T171765 WP_000615983.1 NZ_CP060963:3758397-3758675 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171765 NZ_CP078110:c2549855-2549508 [Cronobacter sakazakii]
ATGTGGACTATTTACCTTACTCACGAGTTTGAGTGCTGGCTCGCGGGACAGCCGCAGCCGTTGCAGGAAGATGTCCTGGC
CGCGCTGGGGCTTTTGAAAATCAAAGGTCCGCACCTCGGGCGTCCGTATGCCGATACGCTCAAAGGCTCGCGTCACGCGG
GTATGAAGGCATTACGGGTTCAGCACGCCGGGCGGCCCATACGCGCCTTTTACGCTTTTGATCCGCACCGTTATGCGATT
GTGCTGTGCGCGGCACAGAAAAAGGGCGATGAAAAGCGGTTTTACCGGGTCATGCTGCGCGTCGCTGACAAACTGTTTAG
CCACTATCTGAACCACCGGGAGGTATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT171765 WP_000729703.1 NZ_CP060963:3758134-3758394 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171765 NZ_CP078110:c2549507-2549208 [Cronobacter sakazakii]
ATGAAGACGTTTGATGAAGTGCTGGCGGCGCAGACCCCGGAGGCTCGCGCGCGTATCGAAGAGAAAACGGCGCAGCTGCT
GCTGGAAACCCATCTGTATGCGCTGCGCGAGGCGCTCAGCGTGTCGCAAACCGAAATGGCGCGCCAGATGGGTATCGCCC
AGCCGTCGGTCGCGGCTATCGAACAGCGCGGCAATGAGATGAAAATCTCAACGCTTAAGCGCTATGTCGAAGCCCTGGGC
GGCACGGTGCGGCTGGATGTCGAGCTGCCGGACGGCCAGCGTTTCGGTTTTACGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References