Detailed information of TA system TA171764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3751937..3752631
• Replicon: chromosome
• Accession: NZ_CP060963
• Organism: Escherichia coli strain EC7

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H9194_RS18595
• Protein ID: WP_001263489.1
• Coordinates: 3751937..3752335 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H9194_RS18600
• Protein ID: WP_000554758.1
• Coordinates: 3752338..3752631 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3747525)	3747525..3747605	-	81	NuclAT_12	-	-
- (3747525)	3747525..3747605	-	81	NuclAT_12	-	-
- (3747525)	3747525..3747605	-	81	NuclAT_12	-	-
- (3747525)	3747525..3747605	-	81	NuclAT_12	-	-
H9194_RS18570 (3748201)	3748201..3748659	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H9194_RS18575 (3748920)	3748920..3750377	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H9194_RS18580 (3750434)	3750434..3750955	-	522	Protein_3654	peptide chain release factor H	-
H9194_RS18585 (3750951)	3750951..3751157	-	207	Protein_3655	RtcB family protein	-
H9194_RS18590 (3751475)	3751475..3751927	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H9194_RS18595 (3751937)	3751937..3752335	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H9194_RS18600 (3752338)	3752338..3752631	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H9194_RS18605 (3752683)	3752683..3753738	-	1056	WP_001226164.1	DNA polymerase IV	-
H9194_RS18610 (3753809)	3753809..3754594	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H9194_RS18615 (3754566)	3754566..3756278	+	1713	Protein_3661	flagellar biosynthesis protein FlhA	-
H9194_RS18620 (3756502)	3756502..3756999	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T171764 WP_001263489.1 NZ_CP060963:c3752335-3751937 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T171764 NZ_CP078110:c2495056-2494694 [Cronobacter sakazakii]
ATGGCATGGACGGTGATTTTTACCGATCGTTTTAACGACTGGTATCAGCAGCAGCCCGAAGGATTACAGGATCGTATCGC
CGCGCTCTTATGGAACTTACGTTATTCCGGCCCGCTTGTCGGACGCCCGTTGGTCGATACGGTGAAAGGTTCGCGTTATC
CCAACCTGAAAGAGCTACGTGTGCAGTATGGCGGTGAGCCCTGGCGCGTCTTTTTTGCGTTTGACGCTGAACGTCAGGCC
GTTGTGCTCTGCGCAGGCAATAAACGAAGCCAGAAACATTTTTATGACGCCTTAATCAGGCAGGCTGAAGAAGAATTCTT
CCTGCATGTGCAAGCGATGGAGAGACGAAATGAAAACTCTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT171764 WP_000554758.1 NZ_CP060963:c3752631-3752338 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT171764 NZ_CP078110:c2494707-2494411 [Cronobacter sakazakii]
ATGAAAACTCTTGATGACGTAATGGCGGGCTTTTCCCCGGAACGGCAGGCTGAAATTCTGCGCATGGCTGACGAAATCGC
GCTGGAGCACGGCCTGCCGCGCATTCGCGAAGCGCGGGCGTTTTCGCAGCAGCAGCTGGCGGAGATAATGGGCGTGACGC
AGCCCGCTATCGCCGCCATTGAGCAGCGCGGTAAGGAGATCAAACTGCTGACGCTCAAGCGCTACGTCGAAGCGCTGGGC
GGCAAGCTCTCTTTACTGATAGAGCTGCCCGAAGGCAGCAAAGTGATCCCCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3