Detailed information of TA system TA171750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1785778..1786280
• Replicon: chromosome
• Accession: NZ_CP060963
• Organism: Escherichia coli strain EC7

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: H9194_RS08545
• Protein ID: WP_000767829.1
• Coordinates: 1786026..1786280 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: H9194_RS08540
• Protein ID: WP_001259255.1
• Coordinates: 1785778..1786029 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9194_RS08515 (1780956)	1780956..1782023	-	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
H9194_RS08520 (1782023)	1782023..1783093	-	1071	WP_000108933.1	histidinol-phosphate transaminase	-
H9194_RS08525 (1783090)	1783090..1784394	-	1305	WP_001446927.1	histidinol dehydrogenase	-
H9194_RS08530 (1784400)	1784400..1785299	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
H9194_RS08535 (1785445)	1785445..1785495	-	51	WP_001364200.1	his operon leader peptide	-
H9194_RS08540 (1785778)	1785778..1786029	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
H9194_RS08545 (1786026)	1786026..1786280	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
H9194_RS08550 (1786363)	1786363..1787187	+	825	WP_000754749.1	SDR family oxidoreductase	-
H9194_RS08555 (1787233)	1787233..1788162	+	930	WP_241462831.1	LysR substrate-binding domain-containing protein	-
H9194_RS08560 (1788377)	1788377..1788439	+	63	WP_010723108.1	membrane protein YoeI	-
H9194_RS08565 (1788429)	1788429..1789787	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
H9194_RS08570 (1789966)	1789966..1791024	+	1059	WP_000492321.1	thiosulfate utilization transporter TsuA/YeeE	-
H9194_RS08575 (1791038)	1791038..1791265	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T171750 WP_000767829.1 NZ_CP060963:1786026-1786280 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T171750 NZ_CP078093:1662925-1663275 [Crassaminicella indica]
GTGATTGTAAAAAGAGGAGATATTTTTTATGCAGATCTTAGTCCTGTTGTTGGTTCCGAACAAGGAGGGGTAAGACCAGT
TCTTGTAGTTCAGAACAACATCGGGAATAAATATAGTCCAACTATCATTGTGGCAGCAATCACCTCACAAATTAACAAAG
CAAAAATGCCTACCCATGTTGAAATTAGTGCTTCGGAATATGGATTAACAAAGGACTCAGTAGTTTTGTTAGAACAAGTT
CGAACTATTGATAAAAGAAGGTTGAGAGAAAAAATAGGGCACTCCGATGATGAGATGATGGATAAGGTGAATGAGGCGTT
ATTAATTAGCTTTGGATTAATAGATTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT171750 WP_001259255.1 NZ_CP060963:1785778-1786029 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT171750 NZ_CP078093:1662648-1662923 [Crassaminicella indica]
ATGGCTGAGTCTAAACGAATTATGATCAGTTTACCAAATACTCTTTTGGAGGAAGTAGATAATATTGTAGCAATAGAGAA
AAGAAATAGAAGTGAATTTATTAGAGAAGCAATGAAATTATATATTCGAGAAAGACGCAAAATTCAAATAAGAGAAAGCA
TGAAAAAGGGTTATAGGGAAATGGGGGCTATGAATTTAACATTATCAGAAGTAGGTTTAGATATAGATATGGATGTATTG
TCAAATTATGAAGCTAAGTTAGCGGAGTGTGAGTAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0